Hi.

I'm looking forward to start a series of experiments to detect the nature and distribution of some hydroperoxides from methyl linoleate mixed with a pigment. I've read several articles but most of them were published before 2000 (and typically in the 1970s or 1980s), in a time when technology was not as advanced as today.

The goal would be to detect specific hydroperoxides arising from methyl linoleate photo-oxidation, and hopefully being able to tell exactly which geometric/positional isomers I've found (e.g. the 9-OOH or 10-OOH).

Most of old literature uses reduction with sodium borohydride or potassium iodide, and also hydrogenation with a platinum catalyst. Given that I would prefer to avoid too much handling during the experiments and keep the procedure simple as much as possible, what would you recommend me to try, in order to analyze those compounds?

You see, hydroperoxides are generally converted into their more stable hydroxy-derivatives and then sylilated with BSTFA in pyridine or maybe hexamethyldisilazane. Is there any way I can still distinguish hydroperoxide isomers without reductions?

Thanks.