What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

Are you interested in the conference 'Sport and crisis: bodies, practices, representations'?

Dear sport sociologists, you can find below the Call for Papers of the Conference - Sport and crisis: bodies, practices, representations. The conference will be held in a hybrid form at the...

30 November 2020 460 7 View

I can't make my RAPD-PCR work anymore. Can anyone help me?

Guys, I REALLY NEED HELP At the end of the last year I optimized every parameter of my RAPD-PCR, and as a result I did obtain great products multiple times. Then, I went home for my vacation (I...

18 February 2020 9,236 3 View

What else other than protein absorbs at 280nm in my sample when meassuring protein concentration at nanodrop?

Dear all, in our lab we are working on Outer Membrane Vesicles (OMVs). When we purify these vesicles we simply quantify the total protein content using nanodrop. Then we check the quality of the...

26 February 2018 4,800 10 View

Does anyone have or is aware of literature or data regarding the surplus power generation in solar and wind farms?

Hello, I am looking at possible ways to store and use the surplus power generation from renewable energy plants, solar and wind farms above all. I would be willing to collect some data first, so...

02 November 2017 6,830 3 View

How can I remove a low-copy plasmid from E. coli?

Dear all, in my lab we are working with E. coli. A couple of weeks ago we tried to remove a low-copy plasmid (pACYC) from one of our strains by simply growing it in the absence of the selective...

17 October 2017 9,398 2 View

Live/Dead assay on fixed tissue/cells?

Hi all, is it possible to perform a Live /Dead assay on fixed tissue using Pi and FDA or any other dye? Thank you in advance

14 March 2017 5,077 1 View

How can I find out if variable A moderates the effect between variable B and C using SPSS?

I already proved a negative relationship between variable B and C, however my hypothesis in my research paper is that variable A moderates the negative effect of variable B on C. I read a number...

06 December 2016 2,698 4 View

How do endocannabinoids modify the immunohistochemical expression of CB1 (receptor) and FAAH (enzyme)?

The increase of the ligand increases the receptor and the enzyme or only one or the other.

14 April 2013 8,452 3 View

Irradiation data for JAPAN?

Hi, Could you help me to get the latest (2019, 2020) solar resource data for a particular site in JAPAN? PV GIS and NSRDB data are not in reach to JAPAN, I'm looking for some other database for...

03 March 2021 961 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

For modelling epoxy-based matrices (in composites), which model in ABAQUS is the most suitable, Concrete damaged plasticity or Drucker-Prager model?

I am working on developing a micromechanical FE model for predicting kink-band formation in UD composites. To model matrix plasticity, which model, out of Concrete Damaged Plasticity and...

28 February 2021 5,415 1 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Quantifying leaking DNA from bacteria - what's the secret?

Hi, all! I need to determine if my treatment causes membrane damage in bacteria (S. aureus and E. cloacae), but I cannot use a fluorescent probe such as PI and similar ones because my compound is...

24 February 2021 2,006 2 View

How the Ground Plane implantation is performed without damaging (and w/o varying the undoped nature) the top Si (8-10 nm) in FDSOI technology ?

It is a general practice to introduce Ground Plane in FDSOI devices under the BoX. The Ground Planes are introduced by Ion Implantation through Top Si layer and Buried Oxide. I wanted to know that...

24 February 2021 5,974 4 View

How to Control Whiteflies in coconut leaves?

24 February 2021 1,005 1 View

Any references about insects or animals that can damage or weathering of rock or fossil material?

Are there any references to insects or animals that can cause damage or weathering to the rock or fossil material? books or paper that would be nice. Thank you very much!

24 February 2021 6,805 3 View

I have a time series data of some biochemical studies ( DNA and Chromosomal damage) which I intend to use to further predict into future ?

I have a time series data of some biochemical studies ( DNA and Chromosomal damage) which I intend to use to further predict into future without necessarily conducting the assay for an extended...

23 February 2021 7,842 1 View

How to solve the fatal error "the entire source was rejected" in MCNP?

Simple geometry was made having concentric cylinders bounded by a square region. The geometry has a reflective boundary. The source was defined at one point in the boundary and when it had been...

17 February 2021 4,364 3 View