How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

No title

03 March 2021 5,360 2 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

The procedure compute?

02 March 2021 5,204 3 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

Which transition metal salt precursor is best for doping? Why?

I would like to synthesize a transition metal doped TiO2 via solgel method. Hence, I would like to know which metal salt precursor to choose among Chloride, Nitrate, and Sulphate. A clear...

27 February 2021 4,285 3 View

When I reacted Bidentate NS Schiff Base with metal salt (M-Cl2) in +2 oxidation state in proportion of (2:1) , getting sticky product ,, why?

When I reacted Bidentate NS Schiff Base with metal salt (M-Cl2) metal chlorides in +2 oxidation state in proportion of (2:1 Schiff base &metal salt) , i was get sticky material Why ? ---~...

21 February 2021 7,970 5 View

Which non-coordinating solvent dissolves anhydrous Iron(II)chloride?

Hallo, i am looking for a non coordinating solvent that dissolves anhydrous FeCl2 for a synthesis in which i can not use solvents like thf, pyridin etc.. I already tried benzene (no solubility),...

11 February 2021 6,105 1 View

Do we have any empirical model to model the combined effect of carbonation and chloride attack in concrete concerning climate change?

Do we have any empirical model to model the combined effect of carbonation and chloride attack in concrete concerning time-dependent temperature, relative humidity and concentration of carbon dioxide?

08 February 2021 4,277 1 View

Selecting referencing electrodes for electrochemical experiment?

I have several questions, What reference electrodes is commonly used for aqueous electrochemical systems? What reference electrodes is commonly used for electrochemical systems which used...

06 February 2021 6,389 5 View

In Michaelis-Menten Kinetics, why K(subscript)On have units (mol^-1)(time^-1)?

where K(subscript)On is the rate of Enzyme (E) + Substrate (S) going to Enzyme-Substrate complex (ES). I think I understand why K(subscript)Off and K(subscript)catalysis have units of time^-1....

11 January 2021 649 8 View

What is the mass% and mole% of water in the 11ml solution?

10 January 2021 127 2 View

Can anyone provide me with a procedure for determining chloride ions in soil samples?

I want to test the chloride ion concentration in the soil sample where I am conducting a research, but I cannot find a procedure to proceed. Thanks in advance!

07 January 2021 5,646 9 View

I am trying to turn a carboxylic acid into an acid chloride. I plan to use thionyl chloride for this but is there something less dangerous?

I need to convert Sodium butyrate into an acid chloride for my work. I've perused the literature but have not found too much about the details of these types of reactions. I plan to use thionyl...

21 December 2020 270 3 View