How to minimize the standard deviation of protein-ligand complex using mmpbsa (CaFe 2.0 vmd plugin) using namd?

Hello, I wanted to know how can I minimize the standard deviation (SD) of my simulated protein-ligand complex of 50ns using MM/PBSA method of Cafe 2.0 plugin of VMD plugin using NAMD. I have tried...

10 December 2020 8,132 1 View

What is the configuration file for blind docking using PyRx for multiple ligands?

I am trying to run blind docking with multiple ligands and one macromolecule using PyRx but somehow i am getting poor binding affinities. What is the configuration file for blind docking in PyRx...

01 April 2019 2,132 3 View

How to deposit gold nanoparticle onto (commercial) disposable screen printed carbon?

i have carbon screen printed electrode and gold nano particles suspension in citrate buffer, how to deposit the particles on the electrode in a way to get uniform distribution?

28 February 2019 9,323 3 View

If i have protein amino acid sequence how to get the 3D structure?

i have amino acid sequence of protein and I am looking for a free tool can predict the 3d structure, also i have the aptamer of this protein and i want to know how they bind with each other ?

16 February 2019 5,364 5 View

What is the basic different between IDA and SPE?

Currently Iam working on amperometric biosensor using SPCE (screen printed carbon electrode) but I still confuse to figure out what is the different between this electrode with Interdigitated...

04 November 2018 7,738 2 View

How to design read out circuit for electrochemical sensor?

hello, iam working on an electrochemical sensor, to detect protein biomarker using DNA prob, i want to design a read out circuit for my sensor, also i want to know what is the differantce between...

03 November 2018 2,763 2 View

HMM for maternity dataset using R?

I have attached the simplified form of my data. The variable "Gender" will be the hidden state in our model and "Pregnancy Outcome" will be the observation. What is confusing me is that I don't...

18 September 2018 3,527 1 View

What is the best way to do sample size calculation?

I am conducting a research study and for sample size calculation, i chose a paper with a similar study design to enter measurements in the sample size calculator. Is it correct to choose a paper...

03 April 2018 4,846 5 View

How long does it take to reach a stable OCP value in an electrochemical cell?

I am currently working with low alloy steel in order to achieve EIS (OCP) and Potentiodynamic polarisation values. The value of the potentiable does not stabilise even after an hour of immersion...

18 January 2018 2,808 13 View

To model an ordinal response, with categorical and count variables is there anything other than Ordinal Logistic Regression?

26 June 2017 6,778 5 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Compact antenna for the frequency range 1 MHz-5 MHz?

Hello everybody! I have a problem with developing a compact antenna for the 1MHz-5MHz frequency range. Tell me what type of antenna can be used and what books and articles to read to perform this...

27 February 2021 7,710 4 View

IF troubleshooting: looking at an intracellular protein that localizes to the cell's and how to reduce increase in signal due to cell ruffles?

I am currently trying to stain for PI3KBeta in MDA-MB-231 cells. My current issue is that 1) beta stains all over the cell and 2) there is an increase in signal at the edge of the cell and this...

24 February 2021 8,582 1 View

Does PHA-P stimulate the proliferation of lymphocytes from mice?

Hello, I will start performing MLR using lymphocytes from mice's spleen and I would like to know the better way for the stimulation. If is using PHA-P or CD3/CD28? I would like to know as well if...

23 February 2021 5,476 4 View

Can I perform two way ANOVA in case of some missing groups?

I had control cells and performer ELISA analyses group (time 0). I stimulated cells with substance A and B and cultivated for 1, 2 and 3 hours. On each cells I performed ELISA. I have results...

17 February 2021 8,933 4 View

Books on Monte Carlo methods?

Hi everyone. I took a basic course on Markov Chains, and know a little about Monte Carlo Stimulations and Methods. But I never got to the part of spreadsheets. If anyone can help direct me to a...

13 February 2021 5,261 3 View

Can you realize "top-down" and "bottom-up" ARE THE SAME THINGS at important junctures IN ONTOGENY (child development)?

Can you realize "top-down" and "bottom-up" ARE [ or certainly can, if not MUST, be ] THE SAME THINGS at important junctures IN ONTOGENY (child development)? This Question is NOT addressing YOU...

09 February 2021 5,289 1 View

Are there any compounds that can increase the viability and stimulate cell viability and cell division?

I have trouble growing a cell line that I had just purchased from a cell line distributor. The cells I bought were produced in 2016 and when they were seedling they couldn't grow. There were some...

02 February 2021 3,751 5 View

Buffer for protein solubilization after acetone/AS precipitation that is compatible with cell stimulation?

Hello! I am precipitating some proteins (with either acetone or ammonium sulphate) and I need to resuspend the protein pellet in a buffer that properly keeps the proteins soluble AND which is...

27 January 2021 6,958 4 View

Does the pre-synaptic neuron location in a cortical map influences the spatial position of its synapses in the dendrite of the downstream neuron?

Are functional maps in the cortex used by the brain to carry out computations or are they just a byproduct of wiring minimization? A key element to answer this question is to know if, when...

26 January 2021 1,118 8 View