Hello all,
I am looking for an appropriate explanation about the effects of sequencing platform on downstream analysis of RNA-Seq data.
Here are the facts:
1) A RNA-seq data generated from Illumina GA II platform
2) A RNA-seq data generated from Illumina High-seq platform
3) A RNA-seq data generated from Illumina Nova-Seq platform
3) A RNA-seq data generated from Illumina Mi-Seq platform
4) There may be read length variation among the datasets generated from same sequencing platform
5) What if we do have reference genome available?
6) What should be the better approach to go for de novo transcriptome assembly? whether go for separate de novo assembly or the data-sets can be merge to get a combined de novo assembly?
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