I want to perform an in-vitro digestion on liposomes suspension(used to encapsulate plant extract) and liposome encapsulated in alginate beads. In order to stop enzymatic activity it is reccomented to snap-freeze or use protease inhibitors. In my case, lipase and colipase activity are more relevant as there are no protein in my samples. I wonder what would be the best option, are there lipase inhibitors that are affordable. Since I want to check particle stability using microscopy technique, freezing may not be an option as liposomes are destabilized. I wonder if centrifugation to pellet liposomes/beads would be sufficient, given that enzymes remain in solution.
Thank you in advance
There is a commercially available inhibitor of pancreatic lipase called orlistat:
https://www.sigmaaldrich.com/catalog/product/sigma/o4139?lang=en®ion=US
The following paper may be useful.
Kanwar SS et al. Methods for inhibition of residual lipase activity in colorimetric assay: a comparative study. Indian Journal of Biochemistry & Biophysics 2005;42(4):233-7.
https://pdfs.semanticscholar.org/b2e7/31c0a43d3011d02dfe2ee27b30aab84a3cd5.pdfhttps://pdfs.semanticscholar.org/b2e7/31c0a43d3011d02dfe2ee27b30aab84a3cd5.pdf
https://pdfs.semanticscholar.org/b2e7/31c0a43d3011d02dfe2ee27b30aab84a3cd5.pdf
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