I read some research articles and they are using 80% TFA to dissolve peptides for reaction. can we do the same with proteins or not?
small peptides can be solved in tfa, also a lot less than 80% if there is at least a free N terminus, that is protonated with TFA. I fould this nice article on nature , you can read, https://www.nature.com/articles/174509a0
I don't think so. It is a strong acid hence it will denature the protein.
Acid catalyzed hydrolysis.
It is a really harsh environnement.
OK if you want to denature the protein for analytical purpose.
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