Maybe someone can help me decide which moth family to choose as a good bio indicator.
Hello, Dear friends, I am surveying as part of my thesis research and would greatly appreciate your participation. The study investigates how social media influences dental health behaviors...
01 August 2024 9,349 2 View
Hi, I am learning to perform footprinting experiment. In the protocol I have there are the following components of the reaction buffer: HEPES buffer (7.8 pH), glycerol, BSA, DTT, KCl, MgCl2,...
28 May 2024 1,317 0 View
I'm having difficulty finding a possible solution to my problem: I have a plasmid encoding a protein. In the nucleotide sequence of this protein, I need to add a nucleotide sequence (highlighted...
25 May 2024 6,838 3 View
I know that to many of you this might seem like a really stupid question, but I am a beginner with PCR. If I have a DNA sequence (for example, a plasmid) into which I want to insert a small DNA...
23 May 2024 3,668 1 View
Probably this question seems fool to you, but I am quite new in the research area and there are a lot of experiments I have never done. My question regards the amount of buffer, enzyme, incubation...
15 May 2024 3,993 1 View
I know that depends of the person, but i don't understand why. I would thank you answers
20 March 2024 8,037 2 View
I attempted to clone a small DNA insert (~100 bp after digestion) into a plasmid of unknown size, which originally carried an insert of 111 bp that I removed by double digesting the plasmid. After...
14 February 2024 2,797 3 View
Hello everyone, I am currently working on expressing a protein in Rosetta 2. After harvesting and freezing the cells, the next step is to purify the sample. The initial step involves resuspending...
15 January 2024 9,843 3 View
I inoculated 20 mL of Rosetta pre-culture into 1L LB broth supplemented with 1 mL of Kanamycin and 1 mL of Chloramphenicol. After one hour of incubation, I checked the OD, and the value observed...
13 December 2023 4,927 1 View
Hello, For the systematic review I performed a search using the same strategy on both Pubmed and Embase. I was expecting that Embase would yield more articles. However I got almost double...
23 November 2023 3,413 2 View
I am researching the quality of basic services in the rural barangays of Virac, Catanduanes; and I need additional related literature on the local setting of the subject.
20 July 2024 4,621 0 View
Indonesia has problems related to the continuously increasing level of national electrical energy needs In fact, the electricity consumption level of Indonesian people is higher than other...
08 July 2024 3,120 0 View
Need for a research asap.
05 July 2024 4,990 0 View
Hello! I am currently situated in Paraguay, where I did research on howler monkeys. I am now in the data analysis fase of my project and pretty stuck. Background of the project: I played 5...
19 June 2024 5,319 2 View
I am curious how should i label species in a certain swamp habitat, since i want to make a difference between the migratory and the "stationary" species. Both of the terms i mentioned in the title...
11 May 2024 3,216 5 View
This research question delves into the intriguing relationship between the spread of myxoma virus and various ecological factors influencing wild rabbit populations. By exploring how climate,...
15 April 2024 3,600 1 View
As best I have been able to determine from reading literature, chimpanzees, gorillas, bonobos, and orangutans have been largely confined to tropical and subtropical forests, at least during the...
15 April 2024 2,568 0 View
In the research article "Challenges facing blood transfusion services at a regional blood transfusion center in Western Kenya" by Kavulavu et al. (2022), the demand and need for blood in Kenya...
09 April 2024 1,531 3 View
Antimicrobial resistance (AMR) is a growing concern globally, particularly in third-world countries like the Philippines, where access to healthcare resources may be limited and antimicrobial...
08 April 2024 6,343 3 View
In the study by Kavulavu et al. (2022), they stated that certain sub-Saharan African (SSA) countries have limited financial resources. Data have shown that the situation can be mitigated through...
07 April 2024 199 3 View