Hey,
This may be a odd question, but I am having trouble with the ddRAD Ligation Molarity Calculator Sheet by Peterson et al. (2012) using the BioAnalyzer data.
Can anyone tell me what to enter in the following green shaded cells?
Concentration [pg/µl] (mass/volume concentration from bioanalyzer ("Conc. [pg/ul]")
molarity [pmol/l] (molarity concentration from bioanalyzer ("Molarity [pmol/l]")
annealed adapter conc [pmol/ul] (Final concentration of annealed adapter stock)
What is the difference between the molarity from BioAnalyzer and the Molarity of the Final Concentration?
Everything I read confuses me more and more. I thought it might be the molarity of the stock solution used to make the working solution and the final molarity to be obtained in the ligation mixture? Is this correct?
Thanks a lot to the community.
Cheers,
Julia
Hey Junjie,
Thanks for your answer but this was not what I was looking for. The units are clear but what value do I enter in these fields when I measure the adapter stocks with BioAnalyzer or another Device?
You have 200 micro mole of oligos.You annealing them to get a 40 micro mole stock solution, you dilute the stock 1:10 to a 4 micro mole solution and from the dilution you wanna prepare the working solution with the sheet of Peterson et al. I measure the 1:10 on the BioAnalyzer and what values do I enter in the cells.
Please correct my procedure, if I am wrong.
Thanks a lot.
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