What techniques could be used for quantifying the amount of zinc atoms in a protein?

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Does addition of IPTG before and after lowering induction temperature affect protein expression levels?

Dear all, I would like to know whether addition of IPTG before (37 degC) and after lowering induction temperature (16 degC) affect protein expression levels? *add IPTG before = add IPTG when 37...

17 October 2018 9,027 6 View

Can DTNB displace zinc bound to cysteine residues?

Dear all, My aim is to determine the number of zinc atoms bound to a protein. I came across a technique called PAR/PMPS assay which is quite accurate in determining the zinc-protein stoichiometry...

06 November 2017 2,480 1 View

How to purify oxidation prone proteins?

Dear all, I am currently purifying a small protein which contains 5 cysteines. The purification flowis: Ni-NTA -> His tag removal by TEV cleavage -> remove TEV protease by Ni-NTA column...

01 March 2017 3,722 3 View

Why is my expressed protein differs in size in different host cells?

Dear all, I am expressing a virus protein about 9 kDa on a 16% tricine gel (150V constant). I induced protein expression with 0.5 mM IPTG and collected the cells pre- and post- induction. The...

11 May 2016 6,632 6 View

How do you separate Ni-NTA beads from cell lysate?

Dear all, I accidentally mixed Ni-NTA beads with E. coli cell lysate but has a problem with separating them. Is there any way to separate them so that I can regenerate Ni-NTA beads for...

26 April 2016 6,164 5 View

In CRISPR-Cas9 genome editing, not all genes have PAM sequence, so how do they overcome this?

As the topic implies, when we perform CRISPR-Cas9 genome editing, PAM sequence located after the gene/DNA sequence of interest is essential. However, not all DNA sequences/genes have PAM sequence...

09 February 2016 3,325 4 View

What is like to be a structural biologist in a pharmaceutical company?

I saw some structural biology positions in many pharmaceutical companies, but what do they actually do? Is it the same as doing research? Just curious. Thank you in advance for all the answers. :)

07 February 2016 3,482 1 View

I am having problems with biotinylation consistency, does anyone have any suggestions/advice?

I am biotinylating my monoclonal antibodies at the moment. However, I have biotinylated two batches of them and the reactivity & background(non-specific binding) seems to be...

21 February 2015 2,555 6 View

Separation of organic acids-HPLC?

Hello What should be done to separate and identify organic acids in HPC when their RetTime is the same?Like oxalic acid with Propanoic Acid.or acids that have a very close RetTime.

07 August 2024 8,782 3 View

Absorption coefficient of methane?

Hello, Can anyone provide me with the absorption coefficient of methane gas at 7.7 um? Any reference?

06 August 2024 980 5 View

The aqueous fraction of the hydroethanolic extract is showing the presence of palmitic acid. What is the mechanism responsible ?

Palmitic acid presence in aqueous fraction

05 August 2024 8,624 4 View

How can I add own Henry coefficients in Aspen Plus?

Hi, i would like to simulate an absorption process in Aspen Plus. I want to use the NRTL model und would like to add some individual Henry coefficients. Is that possible and how?

05 August 2024 2,333 2 View

Why is TLC not a form of partition chromatography?

Why is TLC not a form of partition chromatography and paper chromatography not a form of absorption chromatography?

03 August 2024 7,035 3 View

How to convert g/kg Humic acid dose to kg/ha?

I used humic acid at 0.044 g/kg soil in my pot experiment. But finally, I have to recommend kg/ha. Each pot's soil weight was 11 kg. What is the solution?

02 August 2024 7,186 6 View

¿Are oxygen vacancies in semiconductors capable to change the band gap?

Dear all! Oxygen vacancies do raise negatively the Fermi level by increasing the negative charge in the semiconductor crystal structure. In the case of n-type semiconductors, I wonder if this...

30 July 2024 8,339 2 View

How can you draw a coordinate bond between a metal and donor atom in metal complexes, using discovery studio visulizer software?

Hello,, The metal complex ligand appears incomplete in the screen of the discovery studio visualizer

30 July 2024 3,354 1 View

Inquiry on Maximum Nucleic Acid Volume for 2.5 mL Liposome Solution?

I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...

30 July 2024 6,420 1 View

How to make the concentration measurements for the hydrogen absorption/desorption in the metal hydride?

Hello Everyone! I am trying to create a metal hydride system where I am trying to create a temperature control with the emphasis on measuring the concentration of the hydrogen with the hydrogen...

29 July 2024 7,577 2 View