Differences between sheep blood with Alsever solution vs citrated/defibrinated sheep blood in microbiological media?

I am aware of a study that has compared the use of citrated and defibrinated sheep's blood in microbiological media (Hair Sheep Blood, Citrated or Defibrinated, Fulfills All Req... ), but can...

10 February 2021 5,927 1 View

Infinite order of Laguerre?

If Lmn(x) is the Laguerre polynomial of order m and degree n Is there a means of computing Lmn(x) for 1) m goes to infinity 2) n goes to infinity

08 February 2021 6,320 4 View

Sharpening algorithm for similar images with different levels of blurring?

Say for arguments sake I have two or more images with different degrees of blurring. Is there an algorithm that can (faithfully) reconstruct the underlying image based in the blurred...

17 January 2021 7,324 3 View

How to make a pushover Analysis of a reinforced cantilever beam in ABAQUS?

Hello everyone, Im doing a pushover Analysis of a cantilever beam in ABAQUS, made of reinforced concrete, but my model doesnt seem to converge proberly. The program simply aborts before hitting a...

15 January 2021 1,627 3 View

How to illustrate FE Panel Regression with LSDV in a research framework?

First time to use FE Panel Data Regression using LSDV, and not familiar with how to illustrate it in a research framework. We have regional CSR score as IV and Social Development ( four areas:...

14 January 2021 580 3 View

Domestic ancient Egyptian gods and goddesses?

Who are the gods that ancient Egyptians thought of as closer to them and could have a personal connection with them?

25 December 2020 5,505 1 View

Could someone help me recheck my identification of my moth samples?

Greetings to all! I am humbly asking for some help, in regards to the checking of my moth samples. Though it is of great inconvenience to you all, I will respect your decision nevertheless....

08 December 2020 6,009 5 View

Is there a relationship that can be used to relate flow rate (m3/s) to MPa in fluid injection rate?

I am doing simulation exercise of which I have to inject fluid pressure into a borehole. The parameters I am using the injection rate is given in pressure (MPa) and the software accept injection...

01 December 2020 3,221 2 View

What is the current and voltage profile for a short dipole antenna when the monopoles have different lengths?

The textbooks show a triangular pattern for the current as the limiting case for a short dipole, and how this may be considered to be the limiting case for the sinusoidal current distribution....

26 November 2020 5,280 5 View

Best way to teach Data Structures and Algorithms?

Next semester I am teaching an online class on Data Structures and Algorithms. The students will have had two programming classes in C++ and Java each. I am looking for a way to engage the...

11 November 2020 1,176 4 View

Cyclooctatraene damaged by TFA?

16 February 2021 4,747 3 View

Peptide precipitation as pre-step for acid hydrolysis for amino acid analysis?

I'm currently experiencing problems during acid hydrolysis for total amino acid analysis as my first samples contain high amounts of sugar (I get too low results back). I therefore had the idea to...

14 February 2021 6,049 3 View

Extraction of phytochemicals from juice?

I am trying to extract phytochemicals from cane juice. I have tried lyophilization and SALLE techniques but the final product is too sticky and sugars are hindering any further extraction. I have...

03 February 2021 8,784 3 View

How do I dissolve cannabidiol (CBD) and crude hemp extracts in 1:1:18 ethanol:tween80:0.9% saline for IP injection in mice?

I have tried dissolving CBD and crude hemp extracts in 1:1:18 ethanol: tween80: 0.9% saline. I dissolved the drugs in ethanol first then tween, after 10 mins sonication the solutions looks ok but...

27 January 2021 6,259 3 View

What should be the ideal Thiolated-Oligo:GNP ratio for effective conjugation?

I need to conjugate thiolated oligos to GNPs and I am wondering what concentrations of the oligo to use with what concentration of GNPs for effective conjugation.

08 December 2020 7,975 2 View

Can xylose be formed from fructose over acid catalysts?

I am new in the field of sugar chemistry. I wanted to know if it is possible for fructose to be converted to xylose over acidic conditions. I saw in some schemes a pathway from fructose to xylose...

07 December 2020 5,247 3 View

What methods can I use to confirm if my oligo-GNP conjugate is hybridizing with my target nucleic acid?

Once I have oligo-GNP conjugates, I need to use them as sensor for my target nucleic acid. Before doing that I need to confirm whether the oligo probe (conjugated to GNP) hybridizes well with my...

04 December 2020 4,734 2 View

How to solve the problem with protein getting stuck in the wells during electrophoresis for EMSA?

26 November 2020 6,094 3 View

Two - Way Anova SPSS question?

I would like to ask a question about two way anova using SPSS. I want to compare 13 grape varietes (Independent Variable) for some physicochemical characteristics (e.g. reducing sugars which is...

24 November 2020 9,555 7 View

Why my dh5a always flocculated when cultured in M9 medium?

I used the M9 supplemented medium but didn't adjust the pH of 5x M9 salts to 7.2. The final concentration of Ampicillin is 100ng/mL. Here is my recipe: 1X M9 salts 1mM thiamine...

30 October 2020 2,451 1 View