Please help with fluorescence spectra. What substance in wastewater from a building can have fluorescence at excitation 230 nm and emission 570 nm? I see usual protein-like and humic-like peaks, but have no ideas about that one. (Maybe it is yellow fluorescenting protein? No, it cannot be so ;)))
It is almost impossible to guess the substance.
You are monitoring emission more than twice the excitation wavelength so the signal can be simply second order signal. To avoid 2nd order signal use proper filter. 230 nm is too deep UV. Even good quality solvent can have fluorescence impurity.
Is your long-term emission peak (which has a quite poor shape) changing the position depending on excitation wavelength? If yes, it is hardly any fluorescence emission. If not, then, it might some pigment or dye....
Thanks!
I just wanted to check that later (or make emssion scanning). It is not raman scattering. Raman peak from second order of difraction at exitation monochromator is weak and is seen at at about 510 nm.
As far as I know, maximum of humic-like fluorescent peak can shift to the right (whil the peak is getting lower) when ex wavelengths is a bit increased due to variability of fluorescing groups included in himic substances.
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