Can mycoplasma contamination in primary neuronal culture interfere with neuronal growth?

I am doing primary neuronal cultures (mix of neurons and astrocytes) from mice. One year ago we moved and changed labs and since that time I am not able to grow nice neurons (either they don't...

30 September 2020 2,517 1 View

Does anyone have experience with the Menstrual Attitude Questionnaire?

I am considering of using the original one from Brooke-Gunn and Ruble (1980), but maybe there is something better? I am trying to analyze the attitude of athletes towards menstruation and its...

27 August 2020 6,541 2 View

The compensation procedure- beads (for fluorochrome) and cells (dead/live staining)- how to do it?

Hi, I would like to ask about the compensation procedure. I use Fortessa/ Diva and automated compensation procedure. I have a set from BD for compensation setting. Referring to the manufacturer...

28 February 2020 8,765 2 View

What is the best recommended growth and freeze media for SH-SY5Y cells?

I received SH-SY5Y WT cells and started growing them in low glucose DMEM with FBS and S/P. After reading article about changed cell morphology and detachment of cells from growth surface due to...

06 October 2019 1,475 2 View

Why can't I load my purified pcr sample from a gel to another gel to confirm purification The product evaporates out of well before I can run the gel?

23 July 2018 3,700 8 View

Why all phages in ELISA are positive?

I perform 5 biopannings rounds and then did ELISA with the 'soup of phages' from each circle (coating with specfic antybody, then phages and antiM13). Biopannings from 1-4 had really low OD but...

18 June 2018 915 3 View

Can someone help me determine the NMR data for 2,5-dimethoxyampehtamine?

Interpretation of this compound and all the chemical shifts

20 March 2017 703 1 View

Does anybody has an experience with human serum depletion prior to MS- or electrochemical analysis?

12 March 2015 8,710 5 View

Does anyone use a thermo-desorption technique for mercury speciation in solid samples?

22 February 2015 9,352 11 View

How to compute the internal consistency of a scale with ordinal items?

Hi community, I am trying to determine what is the best way to calculate the internal consistency of a newly developed self-esteem scale. I am working together with two other researchers on the...

28 February 2021 1,369 5 View

How can I set up a good fit SEM model in AMOS?

I am trying to make an SEM model in AMOS for my dissertation but having some trouble with getting a good fit. I am looking for relationships between self-efficacy reported on a Likert scale and...

26 February 2021 8,642 4 View

Am i able to conduct a factor analysis on each of my subscales, as opposed to the whole scale?

My psychometric scale contains 38 items in total, with 4 subscales (20, 6, 6 & 6 items respectively? Can i run a factor analysis for each subscale rather than the scale as a whole for the...

24 February 2021 8,856 8 View

Inpatient DBT to reduce parasuicidal behaviour?

Currently trying to obtain as many pieces of research as possible for my dissertation around the implementation of DBT within the inpatient unit for those with a diagnosis of BPD. Primarily...

23 February 2021 5,654 2 View

What are alternatives to factor analysis for a small sample?

My sample size is too small to conduct a factor analysis. I created my own psychometric scale for measuring Effectiveness of Support, which contains 38 items (4 sub-scales) Im really struggling...

23 February 2021 9,287 12 View

Latent profile analysis based on two variables/sources of data?

Hello, for my master project, I would like to conduct a latent profile analysis about self-regulated learning skills. I would like to take two different measures into account when creating the...

21 February 2021 2,326 3 View

How do I collect online measures in a same-different task?

The same-different task requires to subjects to indicate whether a pair of stimuli seen or heard are the same (say AA or BB) or different (say AB or BA). Researchers often collect offline measures...

20 February 2021 9,226 2 View

How many indicators do I need to represent a latent construct?

Hi folks! We did a large survey and planned to use 3 indicators to represent a latent construct (Sense of Place/SoP) in a latent regression model (structural equation modeling). However, one of...

15 February 2021 7,705 3 View

What are some alternatives to the Self-Directed Learning Readiness Scale (SDLRS) developed by Dr. Lucy L. Guglielmino?

The Self-Directed Learning Readiness Scale (SDLRS), also known as the Learning Preference Assessment (LPA), was developed by Dr. Lucy M. Guglielmino in 1977 to measure learners' attitudes of and...

15 February 2021 3,095 4 View

Seeds harvest from white clover by selfing?

I tried to get seeds from white clover by self-fertilization but I could not get the seeds. As you know the white clover is generally fertilized by crossing. I wanna get the seeds by...

04 February 2021 9,152 4 View