Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View

Treating Intermediate Variable in Linear Regression Analysis in SPSS?

While performing the Linear regression analysis in SPSS version 26.0.0. my intermediate/mediation variable goes to the excluded section. Please share your valuable suggestion "How to restrain the...

25 February 2021 4,591 5 View

How is Initial GDP measured ?

Dear All, I am working on estimating Debt-Growth Threshold but confused about some points. My questions are; - How do we calculate initial GDP Per Capita? Shall we do it in STATA or excel file?...

15 February 2021 2,677 2 View

Having trouble with qPCR amplification plots. My curve's never seem to reach a plateau. Any ideas?

The parameters of the curve all seem acceptable, typically reactions results are above 0.9852, -3.1 to -3.6 slope, and 90-110% reaction efficiency. The results seem to be what I would expect from...

04 February 2021 1,138 3 View

How can I convert netcdf file to a binary file in matlab?

Dear all, I have monthly nutrients data from WOA (in a separate NetCDF file) extracted for a specific region. I would like to convert these data directly into a bin file (one of the separate...

30 January 2021 9,241 2 View

How can I get rid of the bubbles in the SBS membrane?

I used methylcyclohexane/ ethylcyclohexane / n-butyl acetate mixed solvent to dissolve thermoplastic SBS & terpene resin. The mixture is then poured onto a glass substrate. After solvent...

24 January 2021 8,306 3 View

Can anyone provide me a protocl of Native PAGE with the 30% terminal resolving gel concentraion?

I am separating the AuCNs, which are Au nanoclusters. I used the 3% stacking and 30% resolving polyacrylamide from the reference. Eachtime when the resolving gel is polymerizing, plenty of heat...

22 January 2021 8,522 3 View

After G actin polymerization and labelling I didn't got filaments. Why?

Purified G actin and got 42kda band and an additional band just below it. While performing G actin polymerization and labeling with rhodamine-phalloidin I didn't observe filaments under the...

20 January 2021 3,751 2 View

How to Import .inp file from abaqus CAE to Ansys perfectly?

I am trying to import an Abaqus .inp file to Ansys but it does not import the whole assembly but only imports a single part. How to solve this.

17 January 2021 5,450 3 View

Which one I should choose in Abaqus ?

Hello, plese give me your idea! I simulate one slab concrete affected by temperature at top and bottom surfaces. after that, have a static load distribution with small area put on top surface. I...

12 January 2021 5,769 4 View

How to remove DCU and HOBT from my reaction?

I am doing a acid and a chiral amino alcohol reaction using DCC and HOBT in DCM or THF? Can anyone suggest me how to remove DCU and HOBT from a reaction mixture??

28 February 2021 2,153 1 View

Questio regarding micro/nano particles fabrication ?

I will start to fabricate micro/nanoparticles for growth factor release and I check out a procedure quite nice, however, it is lacking some details and since I don't have much knowledge doing this...

20 February 2021 199 2 View

How to get phenol(BHT) back to organic phase from acidic aqueous layer?

07 January 2021 6,527 3 View

LC-MS/MS for n- hexane and DCM extract of seeds?

I prepared my seeds extracts in hexane and DCM, now I facing problems in LCMS/MS, it blocks the system, there is any best or ideal mobile phase for both non-polar extracts so that nonpolar...

20 December 2020 2,404 3 View

Precipitation of Pcl from polymeric solution ?

We working on PCL nano particles 1).Ketoprofen (10mg)+ dmso(5ml) Pcl (10mg)+ DCM(5ml) 2).Water 10 ml+ 10 mg When we mix both 1+2 after solublization (400 rpm) Why precipitation of polymer occurs?

09 December 2020 5,033 11 View

Can anybody help me with an efficient way to de-protect poly(tert-butyl norbornene-2-carboxylate)?

I want to make poly(norbornene 2-carboxylic acid), for the sake of characterization, I decided to start with the protected monomer norbornene-tert-butyl-2-carboxylate for the ring-opening...

03 December 2020 3,852 5 View

Can run TD-DFT calculations in two solvent?

I would like the calculation by TD-DFT of the complexes but the problem is this complexes were prepared in DCM/ACN (3:1 , v/v ) Can run td-dft calculations in the two solvent (DCM and ACN) at...

23 October 2020 6,844 2 View

Can TBDMS protecting group be cleaved under 25% TFA/DCM?

I performed a boc deprotection via 25% TFA/DCM (1 hr) without any workup, which should yield TFA salt as a product. My compound also had a TBDMS group on it. But when I took an NMR, the amine peak...

21 October 2020 4,470 3 View

Why is my molecular rotor displaying a bathochromic shift in toluene compared to MeOH or EtOH?

Hello, I am studying the photophysical properties of a molecular rotor which unexcpectedly, shows a lambda(max, absorption) = 565 nm in toluene, but a lambda (max, absorption) = 540 nm in MeOH...

15 September 2020 7,830 3 View

I am having problems in removing m-cpba and its corresponding acid ?

I am doing alkane oxidation using m-CPBA as oxidant in DCM. How can I remove the oxidant and its corresponding acid (m-CBA) from my reaction mixture? I also did aqueous work-up using NaHCO3 and...

13 August 2020 8,290 3 View