When I did my ATPase activity assay , I mixed malachite green reagent on ice, in the ratio of 1:1:2:2 0.572% ammonium molybdate/6N HCl:0.232% ployvinyl alcohol:0.0812% malachite green:ddH2O, but normally it should change color from brown to green for half or one hour, doesn't ? But my MGR reagent keep to be brown , only shows a little lighter after one hour. I don't know what's reason? Can anyone help me ?
The Malachite Green/molybdate reagent, after filtering, is brown and transparent. It turns green in the presence of inorganic phosphate ions.
There is no need to keep it on ice. It is stable at room temperature, but should be stored in the dark long-term.
The Malachite Green/molybdate reagent, after filtering, is brown and transparent. It turns green in the presence of inorganic phosphate ions.
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