I have 3 extract of the same plant.

Aqueous extract - dissolves in saline

Methanol & Ethyl acetate extract - Won't dissolve in saline, needs DMSO.

Now if i decide to do a experiment using these 3 extracts, what should i use as control? Saline or DMSO diluted in Saline?

Should i dissolve all of those 3 extract in DMSO and then use DMSO as control OR dissolve the aqueous extract in saline and the 2 organic extract in DMSO and then have 2 control groups, one with saline and another with DMSO?

Thanks in advance, i did ask a relevant question before but i am still a bit confused.