Hi I am defining populations of spleen and bone marrow based on surface markers, but I am confused with TCR beta, CD69. What population is positive to these markers?
Thanks
Hi Komal,
CD69 is a marker of activated T-cells which would also express both the alpha and beta subunits of the TCR. So I would say double positives would be activated T cells. It may tell you more that that as well but I'm not sure; I'm somewhat new to immunology.
CD69 is a marker for activated T, B, NK and neutrophils, as well as for platelets (http://www.ebioscience.com/resources/mouse-cd-chart.htm). I guess you´re referrring to a double-positive population, so it would be T cells, mainly to be expected in the spleen.
Thanks
if i gate CD69 and TCRbeta separately, i would say CD69+ cells are Activated Tc ells and TCRbeta as ?
In BM you hardly find any T cells, thus virtually all cells should be TCRbeta negative, and the CD69 positive fraction is then the B cells, NK cells and neutrophils. In the spleen, TCRbeta would stain for all T cells with a full TCR (that one is generated in the thymus, if you look in this organ then TCRbeta single positive cells are also immature T cells), but again you also find also e.g. B cells in the spleen, thus CD69 would be a marker not only for activated T, but also B cells in this organ.
In BM, natural killer T (NKT) cells are distributed. I am not quite sure with these panels, they are V beta + (intermediate) and CD69+ (and other NK marker + too, such as NKa.a in B6 background if these are murine samples). So I recommend in the first place you to look into some references that have described about BM NKT cells in PubMed.
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