Protein-aptamer gel electrophoresis help, please??

Hi, I have problems with running gel electrophoresis. I have tried agarose gel electrophoresis and native PAGE. I have two proteins, which have molecular weights of ~30kDa and ~180kDa and two...

03 March 2021 4,275 4 View

Is there any tutorial for comsol general projections?

Hi, im trying to make a projection in a 3d comsol model. I have a non uniforme chanel and my intention is to get the average tempreture in the y-z cross section and se the average tempreture...

02 March 2021 4,624 4 View

Expression cloning by using cDNA,do I need to modify the cDNA first?

I am going to have a expression cloning of mammalian gene by using shuttle plasmid to transforming the E.coli However I don't know I should only inserting the Coding sequence ,or I can...

28 February 2021 5,440 3 View

I need dataset for recommandation system?

Hi every body I need to collect datasets from different source to work on recommendation system algorithms, could you advise me how collect data?

27 February 2021 7,063 7 View

How to estimate 2SLS with different instruments for different endogenous variables?

To be specific, I want to conduct an IV regression in stata with the following three equations. EQ1: Y1 ~Y2 Y3 X EQ2: Y2 ~ Z1 EQ3: Y3 ~ Z2 Both Y2 and Y3 are endogenous. However, Z1 and Z2 are...

23 February 2021 6,266 3 View

Which microtomy technique do you suggest for thin and delicate root sectioning ?And, how to preserve and process the roots before sectioning?

We have thin roots of from 1 year old different species of plants. We are looking into anatomical details of the roots. What's the best method to preserve and process the roots once they are...

23 February 2021 1,898 1 View

How to find out the problem of VMD software when i run it?

Hello I try to install and run the VMD software on windows 10 (64-bit).It doesn`t work and it is closed when I run it. I checked all of tutorials, videos and documents. But they did not work...

22 February 2021 8,211 3 View

Sample modeling in ABAQUS software and its relationship with the real world?

About crack modeling in ABAQUS: Mode I (pure-tensile cracking) and Mode II (pure-shear cracking), these assumptions may be accruing in theoretical approach (ABAQUS) not practical in Lab....

19 February 2021 6,156 1 View

Making my research papers available for readers?

17 February 2021 8,533 2 View

How does a freeze dryer destruct your solid sample?

Several times I lost my sample during freeze drying. There was no solid or powder in the vial after freeze drying. I'm sure sample contained solute because i took its NMR spectrum. I covered the...

15 February 2021 6,749 2 View

How to call both UMAT and UEL together in Abaqus?

I have a model with bulk material (matrix) and inclusions and cohesive interface elements between both. For the bulk material, I have implemented a UMAT and for the cohesive interface elements, I...

20 February 2021 1,286 2 View

Measurement of water retention in organic substrates without a pressure plate?

Hello, I'm trying to measure the physical and hydrological properties of organic substrate media. Currently, I'm referring The book Soil sampling and Methods of Analysis by Carter and Gregorich....

09 February 2021 3,233 8 View

What is the best supplyer for high quality lithium niobate?

Hi, I want the use lithium niobate for the frequency conversion from 1030nm to 515nm. We want to order bulk material and machine it to the required dimensions. There are numerous suppliers of...

07 February 2021 6,786 2 View

What is the best way to improve the working potential window of a solid electrolyte?

My solid polymer electrolyte shows low bulk resistance show quite a low bulk resistance. However, the potential window (obtained from LVS) is very small. Please how can I improve the operating...

31 January 2021 3,340 9 View

How to prepare an algal sample for FT-IR spectroscopy?

Can we measure the bulk composition of biomolecules present within an algal sample directly by placing its powdered form on a FT-IR spectroscopy stage? Or does it require a particular kind of...

30 January 2021 2,303 4 View

Is it possible to culture several cell lines in the same plate/dish/flask with complete cell separation?

I'm trying to screen the effect of a toxic agent. I need to see its effect on a few different cell lines with different degrees of resistance to this compound. I would like to possibly culture...

31 December 2020 5,740 3 View

Vertical distributions of SOC & N is found decreasing with increased value of bulk density, shown in literature, is it uniform for the forest soils?

Almost all the literature shows that the vertical distributions of soil organic carbon (SOC) & available nitrogen (N) is found decreasing with increased value of bulk density with respect to...

21 December 2020 670 10 View

Is grain boundary scattering 'always' dominant over surface scattering in grained metal/semiconductor thin films?

Hi all, I was wondering if there is some theoretical insight in the relative contribution to the resistivity of grained thin metal or semiconductor films coming from bulk grain boundary...

18 December 2020 2,969 3 View

Bulk density obtained of the forest surface soils is 0.81 gm/cubic centimetres, is it normal?

Bulk density of a forest soil sample in a community forest created under social forestry scheme in the district of Nadia, West Bengal is obtained as 0.81 gm/ cubic centimetre, comparatively too...

30 November 2020 4,706 33 View

Can anyone suggest to me a company that produces bulk metallic glass powder?

I'm studying for a master's degree. I'm doing research in 3D printing by using metallic glass powder... I would like to buy some to do experiments. It would be very helpful if anyone can suggest...

29 October 2020 184 4 View