What might be the possible cause of low yield of RNA in Guinidine-Phenol-Choloform extraction methods??

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Does anybody have brain dissection protocol of zebrafish??

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Is there a problem with my RNA pellet?

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Strugglling with m6A dot blot any suugesstion ?

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How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?

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RNA Extraction Using Hot Borate Method No Longer Working?

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Does Anyone have expertise in in vitro transcription and RNA pull down assay?

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E.coli contamination in human RNA seq data ?

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RNA later for the preservation of RNA in fecal samples at room temperature for one day (37°C)?

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The aqueous fraction of the hydroethanolic extract is showing the presence of palmitic acid. What is the mechanism responsible ?

Palmitic acid presence in aqueous fraction

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Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

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Which solvent is better to dissolve with secondary metabolites extracted from fungi?

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Ashraf Abbas

you should work on fresh braim samples if possible also check the ph of phenol and choloform.

Soran Mohammed

Cell lysing step is directly related to a low RNA yield so I would first check whether the cells are lysed properly then think about other possibilities.

Md. Mahiuddin Zahangir

Thanks professor Ashraf Abbas , Soran Mohammed and Jack Greenhouse for your suggestions