Previously i extract RNA from the brain samples and get very high yield. But now the amount is decreasing. what are the causes of low yield of RNA??
you should work on fresh braim samples if possible also check the ph of phenol and choloform.
Cell lysing step is directly related to a low RNA yield so I would first check whether the cells are lysed properly then think about other possibilities.
Thanks professor Ashraf Abbas , Soran Mohammed and Jack Greenhouse for your suggestions
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