What methods are used to accurately test the % lipid content remaining in the femoral heads after processing?

12 February 2015 1 6K Report

We have our own method that we are trying to validate but I just need to know what accurate tests are other tissue banks using in order to test and calculate the % lipid content left behind.

Article Processing of Whole Femoral Head Allografts: Validation Meth...

Jorge Ruiz Carrascal

I think chloroform:methanol extraction procedures specifically developed for biological tissues are the most accepted ones as reference methods. That is Folch method or any of the subsequent modifications:

http://www.jbc.org/content/226/1/497.full.pdf+html

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