I am trying to isolate microorganisms from enriched activated sludge that are able to degrade benzotriazoles. The enriched mixed culture is able to degrade the desired compounds but I failed till now to identify the organism that is responsible for that. Does someone know a good isolation method or a good (solid) enrichment media? I tried R2A, LB, THB, and some other minimal media without any success. Thanks a lot for helping me.
Hi, thanks for your commment but unfortunately the compound only shows toxic effects bejond its limit of solubility in the solution. But your idea with the agar plates sound promising.
you can use N.A media and cover surface with benzotrizoles and streak with isolated bacteria then incubate at 30c for 7 days, if you see hallo zone around colonies, your results positive.
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