I was using autoclave for digesting water samples for Total phosphorus measurement with ascorbic acid method. The autoclave was set to perform 2 hours digestion and around 121 Celsius degrees. I only applied one drop phenol and 0.4 gram of ammonium persulfate to 50 ml water samples before digestion. However, sometimes I discover the water samples turned out become yellow in few bottles after digestion, but (so far) it never happened to standard solution which are digested at the same time. Anyone ever experience the same thing?
Thank you so much for your help.
:)
Phosphorus occurs in natural waters and in wastewaters almost solely as phosphates .When do the autoclaving the phosphorus convert into phosphate Phosphate having the yellow color texture
Kindly check https://www.epa.gov/sites/production/files/2015-08/documents/method_365-3_1978.pdf
Also check https://dnr.wi.gov/topic/labcert/documents/training/TP_NH32.pdf
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