What kits can be used to do co-staining with the IHC method?

More Shibabrata Mukherjee's questions See All

I wish to do TEM in FFPE blocks, can anybody provide me the protocol to proceed with?

I have dermal FFPE biopsy blocks that I would like to do TEM with. To study the ultra structures, what else do I need? Please help me with the protocol and materials required.

06 July 2014 7,779 3 View

Can we use antibodies for frozen sections to stain FFPE samples post antigen retrieval?

I have been trying to do the same for CD28 which is for frozen section, but with no luck. Does an antibody for CD28 FFPE staining exist? I am trying to do the staining in a skin sample from a Post...

05 June 2014 589 9 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View

Reagents for histology slides?

Dear all, I am planning to study histology and want to do that at home. I have only stained a blood smear with Giemsa but now want to try a wider range of animal tissues. What reagents will I...

24 February 2021 5,430 3 View

Does anyone have any experience with the Motic EasyScan Pro Slide Scanner?

I am interested in purchasing a slide scanner for histology and was presented with the Motic EasyScan Pro but I have never heard of the company, nor know of anyone who has used the system. Can...

11 February 2021 2,416 2 View

Having trouble mounting the sections on the slides for IHC staining, what solution should I use?

Hello everyone, I just started learning IHC staining and I have some frozen sections to practice with in a 96-well plate. I'm having trouble mounting the sections on the slide, a colleague told me...

08 February 2021 6,880 3 View

How to fix and protect blood clots for histology ?

Hi, I encounter some problems, I need to my research to fix and cryoprotec some blood clot for histology analyses. Mys current protocol is the following : 48h of para formaldehyde 4% and 7 days of...

07 February 2021 3,756 3 View

Which isomer of xylene do you use in hystology ?

Which isomer of xylene do you use for the preparation of histological tissue sections - meta para or ortho?

02 February 2021 833 4 View

How long and how could I best preserve gill tissue for histopathology?

I have a number of small bodied fishes that I want to extract gills from, so as to do morphological analayses and histopathology. The problem is: I have a lot more that I need to do the same work...

28 January 2021 6,091 3 View

How to do rat bone Immunofluorescence, Please suggest?

Hello, I have been trying to do a rat bone Immunofluorescence histology study. But not able to find a successful protocol till now, Any suggestions? Rat bone: Decalcifies Embedding: Cryo

26 January 2021 4,165 3 View

Antigen retrieval for neural cell marker on frozen brain tissue?

Hi, I'm having problems with the Recombinant Anti-MAP2 antibody from Abcam (Recombinant Anti-MAP2 antibody [EPR19691] (ab183830)) antibody on my frozen, 4%PFA perfused, mice brain tissue. I...

10 January 2021 4,002 5 View

Can someone help me with tissue histology of mice?

I've been trying to assess the efficacy of a drug that I've purified in mouse model. I've induced mammary tumors in balb/c mice and treated them with my drug. I've harvested the tumors, and other...

16 December 2020 3,515 3 View

Maria Cristina Antal

Could you be more precise about your experiment, whether you will reveal the IHC result using chromogens or fluorencence and what king of co-staining you want? (nuclei, cytoplasm, double-immunostaning for a particular differentiation step of keratinocytes). Thank you.

Shibabrata Mukherjee

Dear Mohamed: Yes I want to do double immunostaining.

Dear Maria: I would like to do it with chromogen. cytoplasm and cell surface staining.