What kind of DPBS (with or without iones- calcium and magnesium) is used for immunostaining?. Any answer will be really appreciated.
Froylan
Estoy trabajando en un artículo de investigación sobre el emprendimiento deportivo en Venezuela, y me gustaría contar con referencias similares en los países de América Latina.
29 June 2024 2,980 0 View
La pandemia ha afectado la salud mental de los trabajadores sociales, generando agotamiento y estrés. A largo plazo puede impactar sobre la retención laboral.
11 March 2024 3,779 1 View
Esta pregnta se centra en examinar como los programas de insercion laboral afectan a la integracion social de las personas que no tienen hogar. El objetivo es comprender como el acceso a...
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Esta pregunta investiga la efectividad de las intervenciones en terapia de juego en la mejora de resiliencia en niños en situacion de vulnerabilidad. Se pretende examinar si la terapia de juego...
10 March 2024 7,229 1 View
Greetings. In my line of work DNA extraction is common, i had various experiences with both as buffer TE and water, the former is described as the better way to prevent DNA degradation but...
05 November 2023 1,541 3 View
Greetings. Im searching for protocols for utilization of solid state bioreactors, liquid state are the know standard so information for solid state is more scarce, anyone recomends articles or...
29 October 2023 4,628 0 View
I am currently doing undergraduate work, specifically the methodology part. I want to be able to create distribution models, however I do not know how I could create one based on the distribution...
23 October 2023 3,717 3 View
I need to convert from micromoles per second per liter (µmol s⁻¹ L⁻¹) to millimoles per gram of dry cell weight per hour (mmol gDCW-1h-1) Thanks!
18 October 2023 5,642 4 View
Greetings. Im working with PHB extraction and im interested in the use of ammonium laurate as described in this article, but the article itself is scarce on all details, a general protocol would...
11 October 2023 7,697 0 View
Hello everyone! I am looking for Q1 journals in which it is free to publish in the area of microbiology or pharmacology and I wanted to know if you know of any.
08 September 2023 4,133 4 View
Machine Learning for Automated Diagnosis of Skin Cancer with Dermoscopy Images
21 July 2024 5,471 0 View
Hi everybody, I wonder is there a way to prepare foam from iso-propanol/water mixture? The content of this alcohol should be 70 – 80 w.%. I plan to use silicone-based surfactants, I have several...
15 July 2024 3,539 0 View
Dear all, We've been doing transesterification with our raw materials - a mixture of pairing oil (which is said to came from the skin part of a coconut) and crude coconut oil. During the addition...
14 July 2024 2,200 0 View
I'm looking to immunostain some brain tissue collected previously. The animal was *not* perfused prior to tissue collection. Will the presence of blood interfere with all staining methods...
14 July 2024 177 4 View
Hello, I isolated total histones successfully from the skin primary cells by acid extraction. I wanted to detect histone H1.2 expression in different conditions by western blot. Can some one...
10 July 2024 9,165 1 View
Occasionally we observe lack of staining in the inner part of a skin section processed with indirect immunofluorescence. Can be due to incomplete removal of the cryoprotectan during the wash? Any...
07 July 2024 8,200 2 View
Please explain the protocol in detail along with the reagents to be used
03 July 2024 876 1 View
Hello, I have been performing Western Blots to study the protein levels of a rodent skin and muscle sample. However, I have not been able to produce homogenous reference bands. I suspect that...
26 June 2024 5,913 3 View
Hello all, In ISIC 2016 for skin cancer, how can I tell if 00 or 10 in the attached link is benign or malignant? I know from the image ratio that: 00 is benign and 10 is malignant. But is this...
30 May 2024 3,636 0 View
I am unable to move the way I have. "FORCED DECLINE IN ACTIVITY AND QUALITY OF MOVEMENT. I have noticed "skin feels more like fat than muscle"; I have not been sexuallyvactive, and have had...
26 May 2024 1,593 0 View