Here is the composition of a culture medium named BM medium. It doesn't contain carbon source and nitrogen source, while after cultivation something grow on that. Could anyone explain what bacteria can grow on this medium?
This is not true: BM medium contains a source of carbon, which is agar, as it contains carbohydrates↓
Agar consists of a mixture of two polysaccharides: agarose and agaropectin, with agarose making up about 70% of the mixture.[18] Agarose is a linear polymer, made up of repeating units of agarobiose, a disaccharide made up of D-galactose and 3,6-anhydro-L-galactopyranose.[19] Agaropectin is a heterogeneous mixture of smaller molecules that occur in lesser amounts, and is made up of alternating units of D-galactose and L-galactose heavily modified with acidic side-groups, such as sulfate and pyruvate.[20][18][19]
Agar exhibits hysteresis, melting at 85 °C (358 K, 185 °F) and solidifying from 32–40 °C (305–313 K, 90–104 °F).[21] This property lends a suitable balance between easy melting and good gel stability at relatively high temperatures. Since many scientific applications require incubation at temperatures close to human body temperature (37 °C), agar is more appropriate than other solidifying agents that melt at this temperature, such as gelatin.
This is not true: BM medium contains a source of carbon, which is agar, as it contains carbohydrates↓
Agar consists of a mixture of two polysaccharides: agarose and agaropectin, with agarose making up about 70% of the mixture.[18] Agarose is a linear polymer, made up of repeating units of agarobiose, a disaccharide made up of D-galactose and 3,6-anhydro-L-galactopyranose.[19] Agaropectin is a heterogeneous mixture of smaller molecules that occur in lesser amounts, and is made up of alternating units of D-galactose and L-galactose heavily modified with acidic side-groups, such as sulfate and pyruvate.[20][18][19]
Agar exhibits hysteresis, melting at 85 °C (358 K, 185 °F) and solidifying from 32–40 °C (305–313 K, 90–104 °F).[21] This property lends a suitable balance between easy melting and good gel stability at relatively high temperatures. Since many scientific applications require incubation at temperatures close to human body temperature (37 °C), agar is more appropriate than other solidifying agents that melt at this temperature, such as gelatin.
If not highly purified, agar contained small amounts of impurities. The impurities work as carbon sources. If almost no impurities, Onar's is true and agar digesting bacteria can grow. At this case, surface of agar is embedded. Severe case, solid media become to liquid.
This is not a medium for growing bacteria................so in answer to your question, the answer should be 'not any, really'.
The key to the purpose of this medium is in the name in brackets - namely, it is meant to be for amoebae. Where the BM comes fromin your example, this is unclear, but it looks like a red herring. It's really meant for free living amoebae.
The formula you show is that based on Page's Amoeba Saline (PAS)..................If you do a search in PubMed for 'Page's Amoeba Saline' , you'll also see it described as PAS.
Also previously known as ATCC Medium 1323.
When you add the agar to it, as in this formula, then it can also be called NN (NonNutrient (Amoeba Saline) Agar) .
eg
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