We are doing some experiments for the encapsulation of alpha-lipoic acid into liposomes. For the measurement of Encapsulation Efficiency, we are going to use the UV-Vis spectophotometer. With a spectrum analisys we found that the main peak is at 325 nm. Comparing this result with literature, we found that someone read it from 320 to 330 nm, while others read it at 215-230 nm. Is it possible that this difference depends on the preparation of the samples? Have you got any information or experience about it?
I have attached also many articles in which detection wavelenght is reported in materials and methods.
Many thanks
Hello sir, I have seen ist 3 attachments, in all the cases there is HPLC-UVD. But the mobile phase (or solvent system) used in HPLC analysis is different in all the three cases are not same. The detection wavelength is probably different due to the different interaction of your substrate with mobile phase. But I'm not so confirmed about that. regards.
You can also check the FCC (Food Chemical Codex). A shift in the absorbance due to the dissolving solvent will not be significant.
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