Steve J Sullivan

8 Questions 11 Answers 0 Followers

Questions related from Steve J Sullivan

How long do alexa dyes continue to fluoresce after loading neurons for long-term imaging?

Can anyone share their experience on the lifetime of different tracers or dyes? I am retrogradely labeling neurons with cholera toxin (CtB) 594 for chronic in-vivo imaging. I assume the lifetime...

10 October 2019 707 3 View

What is the typical distance from Bregma to Lambda in adult mouse brains?

I am interested in scaling my coordinates for stereotaxic injections by measuring the distance from bregma to lamda in each of my mice and then scaling this to the "typical" value used in mouse...

11 November 2018 3,793 6 View

What is the typical distance from the surface of the skull to the brain in adult mice?

I am performing stereotaxic injection in mice. In the Paxinos Mouse Brain Atlas it appears that the depth is relative to the brain surface at bregma (z=0). The hole I drill in the skull is tiny,...

11 November 2018 5,429 5 View

What membrane housekeeping proteins (adequately less than or greater than 100kDa) can be used as loading controls for normalization of brain tissue?

I am trying to normalize a 100kDa protein from a membrane fraction taken from brain. Almost all the membrane housekeeping proteins (N-cadherins, integrins, Na/K ATPases) are nearly 100kDa,...

03 March 2016 5,061 8 View

Does anyone know of good retinal ganglion cell specific markers/antibodies that also label dendrites?

I'm interested in immuno-labelling ganglion cells in mouse retina. I am mostly interested in antibodies that would also stain the dendrites (inner plexiform layer), but it's fine if it also labels...

10 October 2014 5,931 10 View

How can I model mEPSCs comprised of single channel conductances in a multi-compartmental cell using Neuron software?

I am interested in using Neuron software to model miniature excitatory post synaptic currents (mEPSCs) built from single channel kinetic schemes. These mEPSCs would contain noise fluctuations,...

10 October 2014 4,725 2 View

Does adding triton with the primary antibody hinder antigen detection in immunohistochemistry?

I've been told that in immunohistochemistry where the detergent triton is used to permeabilize cells, it is best to add triton prior to primary antibody incubation and to exclude it while...

08 August 2014 4,974 17 View

What would be the best method and/or material to encapsulate a protein for later release without denaturing the protein and losing its function?

I am interested in completely encapsulating a protein (to shield it from harsh chemicals) and releasing it, while retaining its function. The protein has a diameter of around 10nm. Ideally the...

08 August 2014 5,894 12 View