What is the temperature required for storage of samples (serum) before HIV, HTLV 1, HBSAg and HCV tests with ECL technique?

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If the emission of all greenhouse gases is stopped now, will the temperature of the earth continue to rise?

Here's a serious question: If we were to stop emitting all greenhouse gases right now, would the Earth's temperature start to cool down, or would the existing greenhouse gases continue to warm the...

30 July 2024 5,123 4 View

How to prepare 100ml solution of 2.5% Glutraldehyde in 0.1M PBS(phosphate buffer) pH about 7, from 25% Glutradehyde and 1X PBS?

final solution volume is 100ml

03 June 2024 417 0 View

How can i use hexamethyldisilazane(HMDS) for drying instead of Co2 and freez drying for SEM analysis of rice anther and pollen?

could you please help with a protocol? should i do it directly on the aluminium stub after dehydration with 100% alcohol or not? should i release the pollen or whole anther is better and dehisce...

03 June 2024 4,793 8 View

What is the best method to enhance the surface hardness of cutting tools made from SPK steel?

SPK tool steels are used for cutting wires made from low carbon steels. We are searching for a cheap and effective method for increasing the cutting characteristics of the cutters.

14 May 2024 4,948 0 View

Constant Reciprocal of Step Size?

Hello I recently encountered a problem with the reciprocal of step size plot in Comsol Multiphysics. After a certain time (seconds) in a time-dependent solver, the plot oscillations will be...

04 May 2024 7,278 0 View

Poor viability of T cell after transduction with CAR Lentivirus!?

Hi everyone I produced a batch of virus , enriched it with centrifugation, and titrated it with T cells after 48h. For my experiments I want to use MOI 3 and that's equal to 1/12 dilution. but at...

02 May 2024 9,727 3 View

Are you interested in submitting papers to a new journal called "InfoScience Trends"?

InfoScience Trends offers comprehensive scientific content that delves into various facets of information science research. This includes but is not limited to topics such as information...

26 April 2024 9,521 1 View

What are these mysterious cells in LA7 CSCs culture?

Hello. This image is taken from la7 cancer stem cells. It seems that the cells that have a purple halo and seen shiner than other cells have a high resistance to starvation conditions. What kind...

13 April 2024 7,831 1 View

Does the color of pan traps affect the sampling of flies from the superfamily Bombylioidea?

Garret, 2020 used traps of blue, yellow, and white colors for sampling and collected several specimens.

06 April 2024 6,995 2 View

Laboratory procedures for identifying the concentrations of formic acid and acetic acid in aqueous solutions???

What are the laboratory procedures for identifying the concentrations of formic acid and acetic acid in aqueous solutions using titration, spectrophotometry, or gas chromatography methods...

06 April 2024 187 3 View

How can I prepare virus for a TEM or SEM imaging?

I have virus (viral hemorrhagic septicemia virus) in suspension and the experiment will not involve cells. What level of TCID50 is preferred?

11 August 2024 3,115 1 View

How to Compress Information Neurally?

Samuel Morse, the inventor of the Morse Code, understood that certain letters in the English language occurred more frequently than others (Gallistel and King 2010). To deal with this, Morse used...

01 August 2024 4,456 2 View

CHO-K1 suspension adaptation protocol?

I am trying to adapt adherent CHO-K1 cells in F12K+10%FBS to suspension culture in only F12K, but in vain. I have followed Mirus bio as well as Thermo protocols, please suggest any alternative....

31 July 2024 3,756 0 View

How to prevent hemolysis after performing cardiac puncture to draw blood from mice in serum?

We have encountered an issue with the serum of mice being hemolyzed after the process of centrifugation. Could you please provide guidance to prevent hemolysis ?

29 July 2024 8,402 4 View

What are the strategies to Enhance IgG-Producing Plasma Cells in mice for Monoclonal Antibody Development?

I have immunized BalB/C mice with a protein using the intradermal (ID) method with Complete Freund's Adjuvant (CFA) and Incomplete Freund's Adjuvant (IFA), following a 14-day interval and three...

22 July 2024 9,160 2 View

How can we improve storage conditions of museum objects?

90% of museum objects worldwide are in storage.

21 July 2024 8,038 6 View

ELISA sandwich with two monoclonal antibodies ?

hi, I am facing difficulties in the développement of an ELISA sandwich. I have several experiences in developing ELISA but that’s the first time I can’t find any solution. My test is currently as...

16 July 2024 9,044 10 View

Why is the baseline fibrosis level of HK-2 cells very high?

In many experiments where HK-2 cells were stimulated with TGF-β at 10 ng/mL for 48 hours, I observed that even in the control group, the baseline expression level of fibrosis proteins like FN1 and...

15 July 2024 1,929 2 View

Can I detect the FLAG sequence of my AAV vector by qPCR?

I am treating human cells with an AAV vector encoding for my gene of interest tagged by FLAG. I want to quantify the mRNA expression of my gene of interest via qPCR to discriminate the endogenous...

14 July 2024 8,482 3 View

Intracerebroventricular (ICV) AAV inj.?

I'd like to know if anyone has got experience in intracerebroventricular (ICV) AAV inj. I am looking for a concomitant spread of the AAV including but not limited to the motor cortex and the...

12 July 2024 781 0 View