What is the temperature required for storage of samples (serum) before HIV, HTLV 1, HBSAg and HCV tests with ECL technique?

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High aspect ratio hexahedral mesh optimization solver?

I am interested in evaluating mesh optimization solvers that converge to high aspect ratio unstructured hexahedral grids. In other words, I am interested in a solver that optimizes warpage,...

26 February 2021 7,279 2 View

How to solve the fatal error "the entire source was rejected" in MCNP?

Simple geometry was made having concentric cylinders bounded by a square region. The geometry has a reflective boundary. The source was defined at one point in the boundary and when it had been...

17 February 2021 4,364 3 View

How does a freeze dryer destruct your solid sample?

Several times I lost my sample during freeze drying. There was no solid or powder in the vial after freeze drying. I'm sure sample contained solute because i took its NMR spectrum. I covered the...

15 February 2021 6,749 2 View

How to model Type 1 collagen structure for finite element analysis ?

I have started a project in which stress distribution in Type 1-collagen scaffold is to be modelled. I am thinking of using finite element analysis to model the structure and the loading...

07 February 2021 5,487 3 View

Hey guys, I'm looking for an efficient protocol to transfect patient-derived tumor cells with a high rate of transient transfection?

As you know it's quite tricky to get primary cells transfected at a relatively high rate!

31 January 2021 3,580 4 View

How can I model a vacuum region between a concentric tube in Ansys fluent?

I am modeling a double pipe (concentric tube) in Ansys Fluent. The center tube contains nanofluid, and the region between the pipes is a vacuum zone. The outer tube is used for reducing convection...

22 January 2021 2,781 3 View

What type of plants is better to work in gene regulation control project ?

I Interested in to work in gene network modeling thus I need to know what is future of gene network modeling in agriculture thanks

04 January 2021 6,527 3 View

Is it possible to culture several cell lines in the same plate/dish/flask with complete cell separation?

I'm trying to screen the effect of a toxic agent. I need to see its effect on a few different cell lines with different degrees of resistance to this compound. I would like to possibly culture...

31 December 2020 5,740 3 View

Help with insect identification?

Could someone help me identify the insect in this picture?

31 December 2020 2,109 5 View

How to find the natural frequency of nonlinear model (hyperelastic materials) in ansys APDL?

I'm working on the FE analysis of nonlinear vibration of circular plate in ansys APDL. I considered the Mooney-Rivlin model of hyperelastic materials. So i want to know that how to find natural...

27 December 2020 8,696 2 View

How the parameters (such as Mw, Chemical bonds, polymer bland and so on) affect the mechanic property of polymer (such as storage, loss modulus) ?

Anyone can recommend any books on such topics? Thanks in advance.

02 March 2021 8,457 2 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

How vaccines of covid 19 work?

Millions ask how vaccines of covid 19 be successful and safe, its here to explain generally how vaccine of pfizer work ,mRNA vaccines teach our cells how to make a protein that will trigger an...

28 February 2021 2,723 4 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Anyone that can help with details in phosphopeptides enrichment?

Anyone know the amount of protein before enzymatic digestion for phosphopeptides enrichment of serume sample in Ti-IMAC? Has anyone ever use Ti-IMAC(the material is from Chinese company named...

28 February 2021 7,310 3 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View

Why does intensity drops as concentration increases?

Is there any reason for decrease in fluorescence emission intensity of HSA,BSA when increase the concentration ?

28 February 2021 3,653 4 View

Can Ethovision store the generated data and video recordings directly to NAS system?

The size of data generated by Ethovision is huge. Having NAS system for data storage seems to be a good option, I am wondering whether Ethovision can directly store the data to the NAS system...

27 February 2021 6,350 2 View

Why is the efficiency of viral mRNA electroporation in PBMC low?

Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...

27 February 2021 9,233 3 View

Recalcitrant seed short-mid storage method?

Dear peer scientists, Ultradry storage is not suitable for recalcitrant seeds. However, theoretically, if we can stabilize the structure of the subcellular membrane, as well as glassify the...

25 February 2021 2,442 1 View