I want to perform calcium flux studies of my formulation on breast cancer cell lines. I found few fluorometric method to achieve it. But I am much more concern to do analysis using Confocal microscopic method.
hi,
have you consider Fura-red or even better Fura-2. Check the Molecular probes handbook (on Invitrogen/ Life Tech) web- site for Ca imaging.
Hi, if you are not attempting to perform precise quantitative measurements (which usaually requires ratiometric dyes like FURA2 or FURAPE3) you should consider using OregonGreen-BAPTA.488. I worked with this dye on a confocal microscope (life cell imaging in neurons) and the results were great (stability, toxicity, bleaching, ease of use and signal to noise ratio). The dye is supplied ready-to use. I would always suggest to perform some kind of (pseudo)calibration with EGTA (chelator) and ionomycin (ionophore) to test the dynamic range and the performance of your microscope.
Good luck!
Thank you Just Genius.
I have not planned for quantitative measurement of calcium. I may think of after getting initial results. Thanks for guidance.
- Badges
- Science topic
- Similar topics
- Social Psychology
- Attitude