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I like to preserve some bacteria culture using stab culture technique but I like to know if I need to add more sodium chloride to my normal LB broth which contains 5g/L of sodium chloride
08 September 2016 5,169 4 View
I need to know the anticoagulant that will not impede PCR.
31 December 2013 9,783 0 View
I need to collect blood sample from HIV patients to screen for torque teno virus and I'm in doubt if using a protein saver card to get the samples will be appropriate as I will need to get out of...
31 December 2013 2,838 0 View
I ligated a DNA size of 3.8kb into topo TA vector. I expected a band size of about 7.7kb from my agarose gel electrophoresis after plasmid purification but got a band size of about 5kb. Pls what...
01 January 1970 6,452 5 View
Dear Researchers I need to know, how to load and plot 2D-PIV (particle image velocimetry) recorded velocity vector field data in Tecplot? Thank You
02 August 2024 3,615 1 View
Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...
31 July 2024 9,892 4 View
I've been trying to clone my N-terminal inserts in the comercial pEGFP-N1 vector. Initially, I cloned my N-terminal insert into a pGEMT-easy vector to ensure that the insert digestion was done...
09 July 2024 4,277 1 View
Hello, recently i am an issue swith cloning where I am using the subclone method to insert my gene length is 1.5kb and my vector(pMAL-c6T) is 5kb I got the colonies which i screened through the...
04 July 2024 7,977 2 View
I am trying to clone a gRNA (23 bases) into the Crispr cas9 pDirect23A vector by using restriction enzyme cloning. I have performed a complete experiment, starting with plasmid isolation,...
02 July 2024 5,448 2 View
I have already cloned ALP gene of L rhamnosus into pET22b+ vector. Cloning is already confirmed by restriction digestion and sequencing. Cloning is achieved in BL21(DE3), Codon+, Rosetta and Lemo...
02 July 2024 5,332 3 View
Hello, I am working on creating a mutant endothelial cell line having Neomycin knock-in present, I have designed my homology arms of 1kb size having neomycin in between them, I would like to...
25 June 2024 1,197 0 View
I am working on a project that involves analyzing remote sensing (RS) images. I need to convert these images into vector data for further analysis. I am looking for automated methods or software...
22 June 2024 6,165 2 View
In my work, Hygromycin B resistance gene is require for screening. I tried to amplify this gene from the vector pIJ941 but failed. I will be thankful, if anyone help me out from this problem by...
19 June 2024 7,822 3 View
Should we use special binary vectors when using plasmid rescue method to detect transgene integration site in plant? that is, there is often no ori site between LB and RB region of simple binary...
28 May 2024 1,873 0 View
