What is the role of Zn cation in the Ca2KZn2V3O12 host lattice, being d10 ion can it show PL emission particularly under the UV light??

More Krishna Bharat Lankamsetty's questions See All

What is the most effective way to sterilize camelina seeds?

I'm working on camelina seed sterilization and have some doubts about the process. I followed a protocol used by my lab mate, but I'm unsure about a couple of things: Is it safe to use 10% bleach...

02 August 2024 1,178 3 View

Research on politics administration relation can conduct based on case study method?

01 August 2024 9,557 1 View

Curcumin encapsulation in Bovine Serum albumin (BSA)?

Hey all what are the ways by which an encapsulated drug inside BSA may escape from its encapsulation. We had synthesised curcumin encapsulated BSA by desolvation method followed by centrifugation...

23 June 2024 8,785 5 View

How does a Man-in-the-Middle (MitM) attack work in the context of Transport Layer Security (TLS), and what specific mechanisms can be employed ?

Context In the realm of cybersecurity, Transport Layer Security (TLS) is widely used to secure communications over a computer network. Despite its robust encryption mechanisms, TLS is still...

19 June 2024 6,595 3 View

Why is colony PCR not working ?

I've been doing colony PCR for last few days but haven't seen any bands on the gel. I've tried both positive and negative controls, but I'm still not getting any bands. Here's my procedure: I use...

07 June 2024 203 2 View

How to prepare 1000 ppm NO3 (nitrate) solution using KNO3 salt ?

I need the calculation procedure for this Thank You

05 June 2024 2,752 2 View

Albumin Nanoparticle Synthesis, troubleshooting?

Hey all I have tried synthesizing Albumin nanoparticles using desolvation technique using glutaraldehyde as a cross linker. After 24 hrs of stirring I could see that the protein has turned brown...

04 June 2024 1,501 0 View

If my standard quercetin equation is Y = 0.0057x + 0.0635 then how can I know the value of ethanolic extract of 10 mg/ml if absorbance value is 0.39?

I wanted to know the value of total flavonoid content in mgQE/gm. Total volume I kept in well plate is 200 µl in which extract was 20 µl. Anyone please help me with this problem

10 May 2024 5,658 4 View

What will be the value of potassium content in mg/100g if absorbance value is 45 ppm in flame photometer?

Amount of fruit powder used 0.15g; final volume of the extract was 100 ml and I have done my experiment in flame photometer. I did not know how to convert 45ppm in mg/100g. Please help me?

10 May 2024 3,173 0 View

Difficulty in Phase separation ?

Hey all I intend to isolate free circulating SiRNA in media using chloroform extraction. I have added chloroform to media containing SiRNA to get the phase separated. The Chloroform came in the...

17 April 2024 8,522 3 View

Which type of compound does lamda max of 218 indicate in a uv-vis spectrum of a partially purified compound through column and TLC?

A crude extract of fungal culture using EtOH was subjected to column and TLC and partially purified compound was obtained. UV vis spectrum of the compound/s has max absorbance at 218nm. The...

11 August 2024 9,801 2 View

Baseline drift in HPLC? What causes this?

Hello, Why do i see this baseline drift when i compare my blank (black) to the sample (blue)? Any suggestions as to why this happened? Thank you!

11 August 2024 3,770 4 View

U you think We need a website software of Blackbody radiation law expert software?

A website software of Blackbody radiation law expert software can used through the following web site. http://39.105.188.151:3000/index

07 August 2024 1,706 0 View

Why is my ASO degraded quickly on agarose gel?

I have been working on Red blood cell-derived extracellular vesicles as Antisense Oligonucleotide (ASO) carriers. We normally run agarose gel to quantify the loading efficiency. I used naked ASO...

06 August 2024 3,130 2 View

Why might the impedance values for DI water and 0.1X PBS buffer solution exhibit a decreasing and increasing trend, respectively over time (HP 4194A)?

Hello everyone, I'm encountering an issue with my electrochemical impedance spectroscopy (EIS) measurements and would appreciate some insights. Experimental Setup: Electrodes: Gold interdigitated...

05 August 2024 3,783 2 View

I'm optimizing a tetra-ARMS PCR protocol with amplicon sizes: 165bp, 123bp and 93bp. Why, in the gel, only 165bp is visible while I'm expecting all 3?

It's an end-point PCR protocol. I'm using 1.5% agarose gel with SyBR Safe dye and TBE as a running buffer, visualization on BioRad XR+ system. I was primarily thinking of primer efficiency,...

01 August 2024 4,673 4 View

Can a Conventional 50W visible LED Lamp Emit UV Light?

I'm researching light sources and their UV emissions. I'm curious if a 50W, 7500K conventional LED lamp emits any UV light. I found that a 200W tungsten lamp can emit up to 1.5 mW/cm² at 350 nm....

31 July 2024 3,843 7 View

Mossbauer spectroscopy Co0.6Mn0.4Fe2O4 MNPs?

Following curve related to Co0.6Mn0.4Fe2O4 Magnetic nanoparticles with extra phase a-Fe2O3. I will appreciate to help me to interpretation this spectroscopy, especially the blue line.

31 July 2024 9,140 2 View

How to calculate conductivity using Electrochemical Impedance Spectroscopy's Nyquist plot?

Hello everyone, I am new to this characterization but watching videos on youtube I have already plotted a Nyquist plot (Z' and -Z'') and already fitted models on my material using Zsim. But now I...

29 July 2024 1,376 0 View

How can we calculate the percentage of configuration interaction (CI) in the UV output data of the Gaussian program?

How can we calculate the percentage of configuration interaction (CI) in the UV output data of the Gaussian program? for example: Excited State 17: Singlet-A 5.1359 eV 241.41 nm...

28 July 2024 9,165 2 View