How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

No title

03 March 2021 5,360 2 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

The procedure compute?

02 March 2021 5,204 3 View

What is the optimum time and temperature of blanching and pre-frying potato for frozen fries?

I am helping a local home industry for developing frozen fries from their local farm. I want to know what is the optimum blanching and pre-frying temperature and time for processing. Please also...

23 February 2021 1,616 5 View

Time taken from pollination to fertilization in solanaceous crops?

How much time is required from germination of pollen grain on stigma to fertilization in Solanaceous crops (chilli, tomato, brinjal)?

26 December 2020 887 5 View

During my work in the laboratory?

During my work in the laboratory, I noticed that fructose, starch and dextrin did not give positive results with their statements such as iodine and sulwanof when prepared 3 to 5 days ago. Why?

25 December 2020 8,390 5 View

What's a cost effective way to make starch based nanocomposite hydrogels in large scale?

starch based nanocomposite hydrogels

13 December 2020 431 4 View

Zeta Potential measurement of starch solution?

I want to measure the charge on the particles in the solutions using zetasizer, but the experiment needs R.I and absorbance co-efficient to be conducted, but i cant find the stated valued for the...

08 November 2020 3,691 9 View

What is the most reliable method for starch digestibility analysis in beans?

I found papers cited Englyst et al. (1992) and mentioned Megazyme kit, But unfortunately these were not clear and hard to follow. Thanks in advance,

23 September 2020 1,476 5 View

Sample preparation for sugar analysis in potato tuber with a refractometer?

For my research it's needed to measure the concentration of the following sugars present in a potato tuber: sucrose, fructose and glucose. Is there a reliable method to measure these with a...

07 September 2020 5,718 4 View

How do I determine the amount of enzyme needed to liquefy a known quantity of starch substrate?

I have an enzyme preparation (powdery form) with an activity of 1045 U/g which I would like to use in mashing a 70 g of grain whose starch percentage is at 67%. How do I determine the quantity...

16 August 2020 1,998 5 View

Which of the food crops is consumed by the largest number of people in the world, wheat or rice?

Presently about forty food crops are in common use in the world over. Amongst these four crops namely wheat, rice, maize and potatoes dominate the scene. However, I want to know which food crop is...

06 August 2020 1,755 19 View

Which methodology is correct to measure Total starch content in wheat in megazyme kit?

I want to measure total starch content in wheat flour. I want use megazyme kit but I am confuse which methodology is correct for wheat because in kit there are mentioned many procedures. Kindly...

23 July 2020 190 3 View