I had studied a number of papers. Different research studies suggest different number of bacterial cells.
I need it for the Kd measurement after 10 rounds of SELEX. Yes you are right for SELEX in first round in some research they started with higher range of CFU/ml later they reduced...
It depends on your bacteria, but based on different studies the minimum number of bacteria should be 1000 cfu/ml.
for more information please check these two papers.
1- https://doi.org/10.1371/journal.pone.0185385
2- Utilizing Cell-SELEX, as a Promising Strategy to Isolate ssDNA Aptamer Probes for Detection of Staphylococcus aureus
Good luck
Negar Bagheri Thank you. I am really obliged with your comprehensive reply.
Aysha Shaukat your welcome, Good luck Aysha
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