What is the reason of getting a false A peak right before every G peak throughout the Sanger sequencing electropherogram?

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Constanza Giselle Taverna

Hi, I am having the same problem at the moment. Did you find any solution to this problem ?. Thanks

Mehdi Montazer

Hi, unfortunately, I could not find a definite answer but I think the problem was probably due to the fluorescence interferences and adopting unsuitable base spacing parameters.

This problem is particularly seen with 'A's and 'G's and shows a spectrum from flanking 'A's and 'G's in true 'AG' sites on one side to the creation of an altogether false A peak attached to a true 'G' on the other side of the spectrum.

Thank you for sharing your experience. I hope you can find an answer soon.

Best,