While Azza M. Al-Derby points you to a variant for staining cells in sputum (1963, staining with Ehrlich's haematoxylin and counterstaining with carbol-chromotrope 2R) I would like to remind you of original sources for Wright(-Giemsa) staining and some possibilities for reading protocols (cf.also  enclosed pdf).

Not knowing which specific type of cells you want to localize with your , I would like to point you also to Lemière et al, 2001: Comparison of cellular composition of induced sputum analyzed with Wright staining and immunocytochemistry, Journal of Allergy and Clinical Immunology108, No 4, Oct2001, Pages 521–523: stating “Agreement between Wright staining and immunocytochemistry was poor for lymphocytes and epithelial cells”.

 But protocols of how to apply and use Wright(-Giemsa) Stains one can find explicitely in (technical) data sheets of suppliers/companies like (number of suppliers not exhaustive, DISCLAIMER: no affiliation to any mentioned company,no financial interests, no recommendations):

EMS-USA ( https://www.emsdiasum.com/microscopy/technical/datasheet/26149.aspx )

MERCK ( http://www.vitus.by/userfiles/file/Products_MERCK/Microscopy.pdf ) Wright's eosin-methylene blue blood smears:Dye mixture for microscopy 25 g Product No: 1.09278; 0.24 g Wright's eosin-methylene blue in 100 ml methanol; leave to stand for 5 days and filter.

POLYSCIENCES ( http://www.polysciences.com/SiteData/poly/Assets/DataSheets/350.pdf )or(http://www.polysciences.com/sitedata/docs/801/2d1cfb49695ef129bbe941ed25feb2fc/801.pdf )

ROWLEY Biochemicals Inc. ( http://www.rowleybio.com/pdfs/B-149%20Wright-Giemsa%20Stain.pdf )

SIGMA-ALDRICH ( http://www.sigmaaldrich.com/life-science/cell-biology/hematology-and-histology/wright-stain.html)

THERMO-SCIENTIFIC ( http://www.thermoscientific.de/search-results.html?keyword=Wright+giemsa&matchDim=Y )

Other stains come into mind:, e.g. Tetrachrome stain, certified (MacNeal) [Blood stain similar to Wright’s stain. Also useful for staining bone sections.], cf.http://www.polysciences.com/Catalog/Department/Product/98/categoryid--66/pageindex--1/productid--262/

Best wishes and good luck.

Addendum to my previous post: another historical blood stain:

GROAT W. A:   A general purpose polychrome blood stain;  J . Lab. & Clin. Med. 21, 978-982, 1936 (cf. attached pdf)

Thank you Azza M. Al-Derby

Thank you very much Wolfgang H. Muss  

I have  Amresco Wright stain its in powder form.

which solvent is most suitable for making solution Methanol or PBS ?

what should be the w/v percentage of wright in solution ?  

Dear Subas Bishwal,

having soome troubles with automatic info-forwarding for new answers and replies via Researchgate I found your answer just recently when joining RG for today. In answer to your questions, - and not to use much space in the answering form - I add here a pdf containing information you perhaps might have no access via Internet.

Regards, Wolfgang

you can see this link:

https://www.emsdiasum.com/microscopy/technical/datasheet/26060.aspx

People search for the answer can read Wolfgang H. Muss attachment. It is clear and easy to understand. 

Here is the protocol for Sigma-Aldrich product :

Fixation:

Streak thin (approximately one cell thick) smears across a sterile slide by means of a second slide or cover glass. Air dry quickly.

Staining:

Place 1.0 ml of the Wright Stain Solution upon the smear 1 – 3 minutes.

Add 2.0 ml distilled water or Phosphate buffer pH 6.5 and let stand twice as long as in step 1.

Rinse stained smear with water or the Phosphate buffer pH 6.5 until the edges show faintly pinkish-red.

For a Giemsa appearance stain 10 minutes in one volume Wright Stain and 4 volumes Phosphate buffer, pH 6.5.

Blot dry very carefully. Stain may be adjusted by further dilution or in the timing of either before or after dilution in the above procedure.

Results:

Erthrocytes yellowish-red

Polymorphonuclears: Nucleus dark purple

Polymorphonuclears: Granules reddish-lilac

Polymorphonuclears: Cytoplasm pale-pink

Eosinophiles: Nuclei blue

Eosinophiles: Granules red to orange-red

Eosinophiles: Cytoplasm blue

Basophiles: Nucleus purple to dark blue

Basophiles: Granules very dark purple

Lymphocytes: Nuclei dark purple

Lymphocytes: Cytoplasm sky blue

Platelets violet to purple granules

References:

Conn, J I Biological Stains, 8th Ed.,Williams and Wilkins Co. Baltimore, 1969.

Clark, G (ed.) Staining Procedures, Williams and Wilkens Co., Baltimore, 3rd Ed., c 1972, p. 122

Link: https://www.emsdiasum.com/microscopy/technical/datasheet/26060.aspx

Thank you Tam for your reply.......

Prepare always fresh specimen- Peripheral smears  from a freshly  drawn blood specimen (EDTA purple top tubes). Allow smears to air dry completely before staining.Please mix the Wright Stain and buffer mixture before blood smears are

made or before the smears are air drying. Combine 15ml of Wright Stain with 75ml of Wright Stain Buffer, mix well and allow to stand for at least 10 minutes. Make sure that a metallic sheen is observed on the surface before staining, Place freshly prepared and air dried blood smears in a manual  staining rack -->Place slide rack with air dried blood smears in Methanol for 30  seconds---> (Adjust timer for 30 seconds--> Take a disposable pipette and flood the Wright Stain on the

appropriately labeled slides. Set timer for 3 minutes. Place oxidizing Wright Stain and Wright Stain Buffer Mixture on Wright stained slides laying on slide rack (Displacing the Wright .Stain off the slides with the pipette filled with Wright Stain/buffer mixture and viewing a metallic sheen on the top of slides.Set timer for 6 minutes. Place slides in Wright Stain Buffer for 1.5 minutes. Set timer for 1.5 minutes. Retrieve slide rack with stained slides from buffer rinse and allow to air dry before examination with immersion oil and 100X objective.Wipe excess stain from back of slides with  methanol soaked gauze, being careful not to wipe off the stained side of the

slides.Place on slide envelope or slide flat/folder and proceed to the microscope room. Place 1 drop of immersion oil onto slide to be viewed making sure

it is completely air dried after staining. 

Dose any body have wright Giemsa SOP for cell (tissue) staining please ,Sigma product

I use Diff Quick.. It is very simple, explanation on how to stain comes explained with the kit. Can be Google and there are videos on YouTube 

To Hamid:

despite the request he posted long time ago I would like to add here for the convenience of others:

go to the Reply from  Tam Quyet Nguyen (this thread, # 08) and you'll find the Sigma-Aldrich protocol....

best wishes and regards, Wolfgang

https://www.emsdiasum.com/microscopy/technical/datasheet/26060.aspx

In honor of  Saeed Vafaei nezhad · 10.48 · 8.34 · Birjand University of Medical Sciences (cf. reply No. 07 this thread) : 

you can see this link:

https://www.emsdiasum.com/microscopy/technical/datasheet/26060.aspx

Aug 22, 2014 · Flag