How to judge and prove the uniqueness of the solutions to the following two matrix equations?

It is about the matrix equation AX=B, where a, b c in A and B are independent elements, X should be made of constants. Case 1 A=[a b 0;0 0 c]; B=[a b 0 0;0 0 c c]; The solution is X=[1 0 0 0;0 1...

16 February 2021 5,698 3 View

Alpha and dense granules in platelets not visible in Transmission Electron Microscopy?

I am trying to visualize alpha and dense granules in platelets. However, it seems like the granules were not stained well. Is there any way I can improve my staining technique to obtain better...

04 February 2021 5,925 9 View

How to improve my BrdU immunostaining?

24 January 2021 414 3 View

Just face a Comsol issue dealing with magnetically hyperelastic material?

Hello all, I am just newly doing a simulation to test the deformation status of a magnetized rubber-like material, which is in-compressible, under a shifting magnetic field. Try to find the...

22 January 2021 7,427 2 View

Any tips on Fab blocking of mouse tissue?

Hi, everyone, I am working on immunostaining of bone marrow and growth plates of mice. And I have to use an mouse monoclonal Ab to do this staining. However, with only secondary goat anti-mouse...

21 January 2021 9,826 3 View

NADP+/NADPH in bacterial cell lysates?

Hello all, we'd like to pick your brain . The enzyme we're testing requires NADPH to work. We have controls showing that our reaction needs our enzyme and our enzyme definitely needs NADPH. Since...

20 January 2021 232 2 View

Any suggestions on 50um cryosection immunostaining (growth plate) ?

Hi everyone I am working on immunofluorescence of cryosection of murine growth plates. The Ab worked well on 8um cryosection. But when I stained 50um section, I found the fluorescent signals...

03 January 2021 9,531 3 View

Do anyone have experience with zymo research yeast plasmid miniprep?

Hello, I am new to yeast research. I am trying to rescue plasmid from yeast and have tried several protocols developed by researchers. But the results are not promising. I ended up buying this kit...

06 December 2020 1,251 2 View

Why are all the cells are stained by EdU when performing EdU assay?

I was wondering whether there is anyone else have this problem? As shown in the images, almost all the cells are stained with EdU, some are whole nuclei (circle 1), some are puncta inside (circle...

02 December 2020 6,911 4 View

Would there loss some bases after the repairment to the viscous ends by using T4 DNA ligase?

In constructing shRNA plasmids, I want to shorten the size of the plasmids, so I want to use two restriction enzymes for restriction digestion, and then use T4 DNA ligase for complement and smooth...

25 November 2020 3,366 2 View

Does anyone know how to plot my station to a section plot on Ocean Data View (ODV)?

I made a vertical section plot with WOA .nc file on ODV, and now I want to plot my stations (csv. file) in it. Does anyone know how to import my points to the section? They are two different...

01 March 2021 3,610 1 View

Is it possible to report hydrodynamic loads on different parts of an ocean vehicle while 6DOF motion analysis in Ansys Fluent is running?

It seems a 6dof UDF containing vehicle characteristics such as mass and moments of inertia can be used for specifying a boundary as reprentative of ocean vehicle. It is required to have...

03 February 2021 7,726 3 View

How can I convert netcdf file to a binary file in matlab?

Dear all, I have monthly nutrients data from WOA (in a separate NetCDF file) extracted for a specific region. I would like to convert these data directly into a bin file (one of the separate...

30 January 2021 9,241 2 View

Why and how to avoid negative values in optical density by spectrophotometry when measurements corrected by blank solvent?

I am trying to assess chlorophyll-a content from coral tissue using spectrophotometry (with SPECTROstar Nano, BMG). However, I always seem to obtain some negative values in my optical density raw...

20 January 2021 1,542 4 View

How to get Argo Data? and is this useful for water column SST data?

I am working on MODIS-SST, I need SST in situ data and water column data, Please guide me in this.

21 December 2020 9,935 5 View

Is there a way to automatically gather surface area from a 3D model based on color/texture?

I have been constructing 3D models of large corals and I was wondering if there is a software that I can use to gather the area of live tissue vs dead/disease? I am aware there is a way by tracing...

17 December 2020 6,991 1 View

Robot that helps clean our seas of microplastics?

My team and I are curently working on a project is focused on preventing microplastics and collecting plastic particles up to 2 * 2mm. Our biggest concern is harming sea life and destroying...

29 November 2020 9,240 5 View

Is there a plausible explanation for the increased frequency of severe hurricanes in the southwest Atlantic Ocean other than climate change?

The Southeastern region of the United States of America, including Florida, Georgia, South Carolina, North Carolina, and the Caribbean archipelagos are being devastated by a higher number of...

16 November 2020 9,463 20 View

What are the environmental variables that influence the Northeastern gray whale population?

I am currently working on my M.Sc. thesis studying how climate change affects the presence of the gray whale in one of its main breeding areas in Mexico. We have considered temperature,...

16 November 2020 8,944 3 View

Why do I receive the sae mesage several times?

26 October 2020 409 1 View