Anxiolytic activity can be assessed using Diazepam as a standard drug and it can be given i.v. Even for furosemide you can use the i.v. injection as a standard. For memory and cognition, piracetam can be used and it can be given orally also. As the treatment would be the same in all the groups, the comparison would be even. I think you should see some of my papers from my profile especially the below mentioned paper:
Nahata et al. 2009. Anxiolytic_activity of Evolvulus alsinoides and Convulvulus pluricaulis in rodent. Pharmaceutical Biology.
Attaching some more links to my papers on the same topic. Hope it helps.
In case of any queries, feel free to ask.
Regards
Article Effect of Convulvulus pluricaulis Choisy. On learning behavi...
Article Effect of Evolvulus alsinoides Linn. on Learning Behavior an...
Article Anxiolytic Activity of Canscora decussata in Albino Rats
Article Anxiolytic activity of Evolvulus alsinoides and Convulvulus ...
Article Cognition boosting effect of Canscora decussata (a South Ind...