What is the optimal temperature of laccase activity in cereals and legumes ?

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Has somebody seen a strange pattern when transiently expressing Prot-GFP fusion in Benthamiana leaves?

Hi, Has somebody seen this strange aggregation before? I don't think they are agrobacteria since they are GFP specific (only excitable by 488 nm laser). Any input is welcomed. Thanks Alaeddine

29 October 2020 2,641 3 View

Does phytase from Aspergillus niger EC 3.1.3.8 GRAS or not ?

There are two origins of phytase : microbial phytase EC 3.1.3.8 and plant phytase EC 3.1.3.26. I want to know if the microbial phytase from Aspergillus niger is GRAS ( Generally Recommended As...

18 August 2016 5,656 3 View

Can anyone help with SDS-PAGE problems?

i'm working on protein evaluation in flour of wheat and others legumes.....I'm using SDS-PAGE. However those days (about two weeks ago) i foudd nothing in the gels (no bands), i dont know what's...

11 November 2020 2,330 3 View

How to isolate proteins solubilized in ethanol?

hi, i'm conducting a study on wheat. I extracted the gliadins from these cereals and now they are solubilized in 60% ethanol. Is there a method of eliminating ethanol (other than boiling) that...

25 October 2020 8,506 3 View

How can you order the clasification in CORINE legends, from a year to another, in type of vegetation?

For example, CORINE clasificate a zone of pasture one year, but in the next temporal clasification give crop of dry cereals, and preview clasification don't find. How can I order then?

20 October 2020 3,913 1 View

Which nutritional component of grains affect the pH level?

Dear community I have a great doubt related to pH in grains. It has been reported that some amino acids could be influencing the pH level. However, could minerals located in the inner parts of...

24 September 2020 6,694 14 View

Does anyone know what is the detection limit of furosine in parboiled Brown rice?

Due to temperature occurred Maillard reaction in parboiled rice and change the rice color. In dairy industry the test of furosine is very common but in cereals its not common. what is the minimum...

18 November 2019 7,618 2 View

Is this a Spodoptera eridania?

These species of caterpillars were found in large quantities feeding on Amaranthus, spinach, water spinach and sweet potato leaves. I have noticed that they also eat legumes leaves. I have...

10 November 2019 5,178 7 View

What could be the possible and most reliable statistical analysis for qualitative traits except cluster analysis for cereals /wheat?

need to know about all possible statistical analysis that can be applied to analyze the qualitative traits or data particular for wheat....

08 September 2019 7,390 3 View

What method can be utilized to determine the amount, ratio, and percentage of trypsin Enzyme required for complete hydrolysis of protein substrate?

I am new to the enzyme and protein field of research. Therefore I am struggling with the following issues- How to calculate the trypsin enzyme amount required for performing the complete...

23 June 2019 5,611 2 View

What method can be utilized to determine the amount, ratio, and percentage of trypsin Enzyme required for complete hydrolysis of protein substrate?

I am new to the enzyme and protein field of research. Therefore I am struggling with the following issues- How to calculate the trypsin enzyme amount required for performing the complete...

05 June 2019 5,679 1 View

What is the importance of legume research in combating climate change ?

Legume and climate change.

04 May 2019 1,358 11 View