When considering phosphorescence lifetime measurements at varying temperatures which aspects does one need to consider when phase change occurs.
Lets say we have a solid sample -> when measuring the lifetime dependence on temperature no problems should arise because no phase change occurs when cooling the sample.
Secondly when a diluted solution is used for lifetime measurements a problem arises, for Example if the sample is dissolved in MeCN -> when reaching temperatures below -60 oC the sample freezes, as it is diluted intermolecular distances between the luminophore molecules should be high enough so they don't interact. Is it appropriate to continue measuring the lifetime dependence on temperature at this point?
This can later be used to calculate zfs in the luminophore between T1 and T3 states to show the energy needed to access the nonradiative triplet state.