Dear Dr. Brandl,

I have used DAPI-staining to determine nuclei number in fungal cells. A small portion of the hyphal mat growing on the surface of the medium were fixed in 2% formaldehyde for 2 min on slide and rinsed with distilled water for 1 min, followed by staining with Gold antifade reagent with diamidino-2-phenylindole (ProLong® DAPI, Invitrogen, USA) for 10 min, and destained with distilled water for 2 min. A drop of 50% glycerin was placed on stained specimen and covered with glass cover slip. Hope it will work for you.

Praveen

Hi,

I am trying to stain with DAPI, but I have a couple of question :-)

I have the fungi plated on media, how do I start? 

How much material do you use for the staining?

Is it only mycelium or is it everything?

When you wash and stain with formaldehyde, the cells much attach to something in order to stay, how to you do that?

Thanks :)

Dear Dr. Praveen Rahi

Can you send me protocol for DAPI staining of nuclei. I have 10mg/ml stock from HiMedia.

Is it possible to use DAPI for staining fungi grown in broth? 

Thanks Praveen Rahi. I stained with EtBr, got good result