How to calculate the annealing temperature of a primer pair?

I have to amplify a gene and my primers just reached. The Tm for Forward primer is 64.2, and that of reverse primer is 65.5. Can some one suggest how to get the best annealing temperature? Thanks...

01 March 2021 360 7 View

How to obtain regions around the person bounding boxes(excluded the detected people in scene)?

Hi all. I have a set of coordinates of bounding boxes for AVA Dataset. This dataset is used in action recognition. My goal is to get the areas around the people (people are inside boxes) and do...

13 February 2021 2,538 2 View

What is the air and water fraction in wave induced spray or splash?

Hello everyone, I was wondering when a wave spray or splash occurs due to wave breaking, what is the percentage of water or air in the volume of spray that we see? I need to have a rough...

21 January 2021 7,738 1 View

How to control tap coefficient (a_n) in microwave photonic filters?

Respected Researchers Refer to the yellow highlighted text, Eq. 6, Fig. 1 and 2 of the attached publication, Please let me know how to control filter coefficient (a_n) mentioned in Eq. 6 with...

18 January 2021 4,724 2 View

Can I publish my patents as research articles, dear colleagues?

Sometimes we have some patents that can also be published as research articles in well-known journals. Is it therefore possible to use your own patented material and publish it as a research article?

11 January 2021 8,399 8 View

Have I add research Q beside research Objective ?

In my thesis should I include both Research questions and research objectives, or I can include research objectives only.

07 January 2021 9,793 3 View

How do we explain the results of the HPLC-MC analysis?

How do we explain the results of the HPLC-MC analysis I need help in how do I read my analysis results of PHLC-MC in the form of a graph? Is there a book or website can help. Thanks

07 January 2021 9,025 2 View

What is Chemical Oxygen Demand (COD) Analysis?

Hello. I want to complete my information on water and wastewater and methods of effluent analysis. What is effluent COD measurement methods?

27 September 2020 2,302 5 View

What is Cox-regression analysis and Kaplan Mier Curves and how can I apply them on SPSS?

SPSS, Data-anaylsis

07 September 2020 3,298 7 View

How to solve the "zero count rate" of dynamic light scattering machine?

Hi, When I start the measurement after putting my sample in DLS machine, it shows zero count rate and didn't measure the readings. I have also attached the screenshot for your kind consideration....

17 August 2020 5,826 3 View

What are the most reliable approaches to extract key continuous frames from a video, with complete automation?

I have an input video, of skin (from a microscope that means it is magnified such that blood capillaries are visible). The idea is to extract 15 sec of continuous frames from a video of 1 min,...

02 March 2021 2,735 2 View

How can extract ciprofloxacin hydrochloride disolve in water er to organic solvent such as dichloromethan or any other solvent?

I need a procedure to extract the ciprofloxacin hydrochloride from aqueous solution by using dichloromethane in detail,. I thank you very much.

01 March 2021 4,306 1 View

Which bacteria are suitable for quorum sensing screening?

I want to find a quorum sensing inhibitor from plant extracts. Klebsiella pneumoniae, Moraxella catarrhalis, Staphylococcus aureus, and Streptococcus pneumoniae. I looked for these strains...

01 March 2021 7,675 3 View

How to perform statistical analysis of coefficients extracted from transform techniques?

I am studying the potential of DCT and FFT coefficients extracted from a signal to classify the different objects that emit the signal. I applied the Shapiro-Wilk to confirm that data was not...

01 March 2021 8,079 2 View

How to remove fatty material from plant extract?

The resultant solid was partitioned three times with 250 ml of petroleum ether (40-60/c) to remove any fatty material and evaporated to dryness.

28 February 2021 9,464 2 View

Can water be extracted from Olivine?

I have always been interested in geology and wondered about how unique structures of materials behave in different circumstances. I stumbled upon Olivine, the magnesium iron silicate, and heard...

28 February 2021 7,330 4 View

How to make fractions of chloroform extract of plant, using seperating funnel?

28 February 2021 8,466 2 View

Good morning research fellows, Has anyone worked with senna alata plant extract on any kind of fish?

Literatures on the piscicidal effect of sennaa alata is scanty. if you have any related litrature either in the bark, leaves or roots used as treatment to any aquatic organism please kindly help...

28 February 2021 9,138 2 View

How to arrange coordinates and extract climate dataset out of MPI-ESM-MR netcdf files?

Have a nice day everyone, I'm stuck in extracting datasets out of MPI-ESM-MR netcdf files because the latitude ranges from -3*10^6 to 3*10^6 and longitude ranges -5,9*10^6 to 5,9*10^6. Plus, the...

28 February 2021 912 3 View

I want to performe MALDI-TOF MS for bacterial identification, I would appreciate it if anyone can tell me the matrix solution preparation protocol ?

based on what I've studied on multiple papers, for peptide and protein identification Alpha-cyano-4-hydroxycinnamic acid (CHCA) is an appropriate matrix to use, I also know that the CHCA powder...

26 February 2021 6,799 3 View