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Ahmad Al Khraisat

stringency :

During nucleic acid hybridization or reassociation, the strictness with which the Watson-Crick base-pairing is required under specified conditions of temperature, pH, salt concentration, etc. Conditions of high stringency require all bases of one polynucleotide to be paired with complementary bases on the other; conditions of low stringency allow some bases to be unpaired.

can see this article below

Article Fluorescence in situ hybridization (FISH): History, limitati...

Article Volpi EV, Bridger JMFISH glossary: an overview of the fluore...