I performed UV-Vis spectroscopy for following samples.What is the right method to find lambda max
Hello, Rishabh Sharma,
it looks like your spectrum does not have clearly visible peak of lambda max, maybe small local max at 295 nm? It seems like lambda max lies under 200 nm. What type of sample do you measure? Some biomolecules or anorganic chemicals?
Martin
Hello, Rishabh Sharma,
it looks like your spectrum does not have clearly visible peak of lambda max, maybe small local max at 295 nm? It seems like lambda max lies under 200 nm. What type of sample do you measure? Some biomolecules or anorganic chemicals?
Martin
I think lambda max is approximately 290 nm according to the response of your samples to UV- Vis.For the method you can use Origin software to get an accuracy measurement.
It is a very bad posted question. I insert it again with my comments and please answer a.s.a.p. Thank you in advance.
I performed UV-Vis spectroscopy (what a kind of spectrometer e.g. single beam, dual beam or diode arrays?)
for following samples
(Please post a picture of your compound (s) (you write samples)
.What is the right method to find lambda max (please give Infos about cuvettes (What a kind e.g. quartz, plastic…) and what a kind of solvent because the cut off of it is very IMPORTANT.
Is your compound pure and dry ? This aspect is important for a correct weighing!!
Hello Mr. Grezes, Sorry for my incapability to ask engaging question. Here it is.
I performed UV-Vis spectroscopy (on Lambda 750 Perkins Elmer, I don't Know kind of spectrometer) for YAG (Yttrium Aluminum Garnet) nanoparticles with distilled water as solvent (cut off near 190 nm if i remember correctly) and cuvette was made of quartz with 10 mm path length.Can you help me now?
The answer was given according only to the obtained results which represented by the curve.
hello Rishabh Sharma,
I send you 2 interesting articles Dong et al and Chen et al.
Please see the UV-Vis spectra and the solvent used in these articles
See p.e. the concentration p. 97 and fig. 5. (Chen et al)
Please note that the solution in your cuvette must be clear without suspension. If you have a suspension of nanoparticles , a broadening of your spectrum occurs.
Good luck
JRG
kindly clear first that spectrum is for organic ligand or ligand-metal complex
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