Some manufacturers do not use nitrogen gas eg : metrohm Ion chromatography. What is the main use of nitrogen gas and also what is the material in the suppressor and inside the column?
CO2 is not only a problem with NaOH eluents. Any alkaline eluent absorbs CO2 and changes therefore pH and eluent strength. In NaOH eluents the elution strength gets higher (CO2) bilds carbonate In carbonate/bicarbonate eluents the elution strength get lower (CO2 titrated carbonate to bicarbonate).
A lot of chromatographers use N2 as prevention from CO2. Initially this was part of keeping eluents degassed. If degassing is no issue (e.g. if eluent degassers are available) then a soda lime tube on the eluent bottle opening is by far good enough to prevent even high concentrated NaOH eluents from CO2
Sir can i use Nitrogen gas insted of Helium Gas for eluents in IEC post column deriv...
Sir I got Lithium Buffer System and it is safe to use Nitrogen Gas for eluent to Pump.....
Kindly Reply someone
- Badges
- Science topic
- Similar topics
- Analytical Chemistry
- Column