In Iodometric titration, sodium thiocyanate is added to prevent the adsorption of iodine onto copper iodide.......................... in the situation where sodium thiocyanate is NOT available, what is the implication of using potassium thiocyanate instead?
hi
Generally if you have an official method of analysis and want to make any change,you should make validation, specially if it is a major change as in your case.
Regards
Dear Mr. Chigbundu, I think that this not a problem. See the links: http://www.titrations.info/iodometric-titration-copper http://archives.library.illinois.edu/erec/University%20Archives/1505050/ch106Brass/titration.htm. It is common to use potassium thiocyanate instead sodium thiocyanate to prevent adsorbtion of iodine on precipitate. Since the reagent is not used to calculate the results of analyte, there is not problem. However, be carefully with quality of the reagent, because contamination with undesirable substances. I hope this helps you. Kind regards.
Using potassium thiocyanate (KSCN) instead of sodium thiocyanate (NaSCN) in a lysis buffer like AVL for viral DNA/RNA extraction can have several implications:
In practice, the choice between potassium and sodium thiocyanate should be based on empirical evidence of their performance in specific applications. If a protocol specifically calls for one, it's usually for a reason based on the chemistry involved and the specific requirements of the process. Substituting one for the other should be done with caution and ideally, validated experimentally to ensure it doesn't negatively impact the efficiency and purity of the extracted nucleic acids.
l Take a look at this protocol list; it could assist in understanding and solving the problem.
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