Can I use TCA instead of TFA for protein analyis in RP-HPLC?

I am trying to analyze lipase using the reverse phase-HPLC C18 column. In the literature, 0.1 % TFA was and 60% ACN was used. Can I use TCA in place of TFA? If any other methods are available to...

25 February 2021 2,388 3 View

What is the difference between Q sepharose column and DEAE Sepharose column?

I am working on protein (lipase enzyme) purification. After using the Sephadex G-25 fine column, which column (Q or DEAE sepharose column) should I use? What are the buffers should I select? Thank you

16 February 2021 6,921 5 View

Why does my MTT Assay remain in purple color and does not change to yellow?

19 January 2021 5,098 4 View

How to use ArcGIS to separate certain % of data points scattered over certain area?

I was able to make a grid using fishnet in ArcGIS. But after that, I need to select 20% of points from each cell and make a different file containing ids of the data points as testing data set....

07 January 2021 9,533 1 View

List of Indian UGC listed journals?

Can anybody help me with the List of Indian UGC listed journals of Science Million thanks in anticipation

02 July 2020 9,671 2 View

How to find out (in-silico) the binding position of miRNA within the mRNA sequence of target gene ??

I have a sequence of miRNA and a large sequence of target mRNA of Arabidopsis and i want to find out the potential binding position of this miRNA within the mRNA sequence. Can anyone suggest/guide...

09 June 2020 7,766 4 View

What GIS tools can be used to analyze the global transmission and prediction of a new coronavirus pandemic, COVID-19?

What GIS tools can be used to analyze the global transmission and prediction of a novel coronavirus pandemic (COVID-19)? What methods and formulas can be used and what kind of geospatial tools can...

14 April 2020 9,225 46 View

Creation of weather data file in Infocrop?

Hai. I am trying to use Infocrop model for climate change effect. I have imported csv file of daily weather data from 1971-2002 into infocrop. It has successfully created the 32 files and CLI file...

15 March 2020 5,446 3 View

How can i calculate the required number of bacterial colonies for a 20kb insert in vector ?

A vector can accept an insert of 20 kb size and the recombinant vector can be replicated in E. coli. In order to make complete gene library of E. coli by using this vector, what is the minimum...

23 February 2020 8,411 1 View

ANN/Deep Learning/CNN etc., are they not overhyped?

ANN/Deep Learning/CNN etc., are they not overhyped? Today under the sun they promise to deliver solution to every problem. But in practise they have limitations. Besides this they are not...

27 December 2019 3,921 6 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

1. What is the impact of having different scales in a survey? and how can we solve this problem before and after data collection (Literature)?

1. What is the impact of having different scales in a survey? and how can we solve this problem before and after data collection (Literature-based reflection)? Thank you for your time and for...

03 March 2021 2,870 3 View

What degree of missing data in a particular case/participant results in Expectation-Maximisation no longer working?

I'm dealing with a mediation model and am using the PROCESS module in SPSS. Due to SPSS and PROCESS being limited in the imputation methods - being unable to handle multiple imputation - the other...

02 March 2021 4,362 1 View

How to access MSCI KLD Stats and Sustainalytics?

Hello all, I'm currently working on my undergraduate thesis and I'm having difficulties trying to access KLD Stats and Sustainalytics because my campus Airlangga University and apparently other...

02 March 2021 8,525 1 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Can NFL theorem be valid in infinite search space in ML?

NFL theorem is valid for algorithms training in fixed training set. However, the general characteristic of algorithms in expanded or open dataset has not been proved yet. Could you show your...

01 March 2021 1,189 3 View

Does anyone know how to plot my station to a section plot on Ocean Data View (ODV)?

I made a vertical section plot with WOA .nc file on ODV, and now I want to plot my stations (csv. file) in it. Does anyone know how to import my points to the section? They are two different...

01 March 2021 3,610 1 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View