What is the shortest time one can sample interstitial lactate with reasonable accuracy? The shortest interval I have found is 45-sec. whilst sampling interstitial potassium.
This would be dependent on a few factors. You would first have to find out the minimal needs for your assay volume. For example, with the CMA600 analyzer (capable of measuring glucose, lactate, etc.), I seem to recall that it only takes 1-2 uL for each sample (2-4 uL in duplicate), and I would certainly recommend duplicate. The next factor would be how fast your pumps are perfusing the tissue of interest (adjustable and portable pumps from CMA can change anywhere from 1-5 uL/min). Keep in mind that as you increase the speed of perfusion (to increase overall sample volume), your relative recovery (concentration of analyte) also will decrease. Hope that helps.
**edited to remove ethanol analysis from CMA600. I should also clarify that sample volume includes dead vol. required and is per analyte measured. So, if you need glucose and lactate, double volume needed per time point.
Joseph,
Thank you for your response, your input was very useful for my future research design.
Cheers,
Nick
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