I believe the range of DCX+ cells in your case seems to be wide. You should use Stereology to count DCX+ cells that will reduce the error in counting and will narrow down the range. Stereology involves optical fractionator method to count the cell density in a specific region and is the only technique available now a days for unbiased counting. After that whatever number of DCX+ cells you get that should should be reliable.

Hello

I have counted DCX cell using stereology technique.  kindly see the attached publication. 

thanks

DCX positive cells are effectively widely distributed in hippocampus. As suggested above, the best way to analyze them is stereology, although you can do it manually.