How to use protein A G beads to purify antibody?

Secretory universal influenza antibody CR9114 were successfully expressed by adenovirus vector ,then we used protein A G beads purify CR9114.But the molecular weight of the purified antibody under...

22 February 2021 4,318 2 View

Amorphous silica XRD peak at around 10 degree?

21 February 2021 3,417 5 View

How could I get the P-value from the mean, SEM, and n of different groups in the t-test?

How could I get the P-value from the mean, SEM, and n of different groups in the t-test without the original data? For detail, I would like to know the P-value between BMP-2: 50ng/ml group and the...

06 February 2021 6,539 4 View

How is linear form of plasmid circularized?

Hi all, I'm working on transforming E. coli with plasmid in a mixture of linear and circular form. What happened to the linear form once uptake? Do cells ligate them directly? Or cell DNase...

13 January 2021 8,336 4 View

What mechanism is involved when chitosan is coated on magnetic nanoparticles?

Hello! I am currently working on coating of chitosan on magnetic Fe3O4 nanoparticles. I am wondering how this coating process happened? i.e. through what mechanism that chitosan can be coated on...

13 January 2021 5,657 5 View

The impact of different poisson's ratios when analysising field vibration?

The Poisson's ratio of soil is quite difficult for us geo-engineers to obtain. I came across a problem when I tried to determine the parameters in a FEM model. For the saturated clay in...

02 January 2021 8,971 3 View

Are there any DNA intercalators that do not have fluorescence?

I understand several normal DNA intercalators, such as ethidium bromide, acridine, SYBR et al. would emit fluorescence. Is there any kind of DNA intercalator that do not have fluorescence?

25 December 2020 688 3 View

Is it possible to deposition a large area dielectric layer(mm scale) on the wet transferred graphene?

I want to deposition a dielectric layer on top of the CVD wet transferred graphene. The purpose of the dielectric layer is to uniformly cover the graphene and enable the top-gate modification of...

14 December 2020 7,693 2 View

Is the fluorescent protein must be totally photobleached in FRAP assay?

Hi I have tried to measure the mobility of a protein of interest by the FRAP assay. The protein was fused with GFP. I overexpressed the fusion protein in Hela cells. However, when the area of the...

14 December 2020 6,567 1 View

Why band gap energy of cobalt ferrites is increased with increased in zinc(dopinng element) concentration?

In most of cases band gap energy of cobalt ferrites is decreased with increase in Zinc concentration that is hold to brass model. But when energy band gape is increases with increased...

03 December 2020 2,439 3 View

Any references about insects or animals that can damage or weathering of rock or fossil material?

Are there any references to insects or animals that can cause damage or weathering to the rock or fossil material? books or paper that would be nice. Thank you very much!

24 February 2021 6,805 3 View

Is this the fragment of a sauropod tooth?

Dear colleagues. Fragments of this species are very rare from a terrestrial Lower Cretaceous locality from Germany. Up to now from this locality sauropods could not be proved with certainty. Other...

14 February 2021 2,310 1 View

Does someone know which structure could exit the olfactory bulbs at their antero-dorsal top ?

Hi people, I am studying bat brains through endocranial casts and I cannot identify which structure could be present in sort of a canal located dorsally to the cribriform plate. It is like...

27 January 2021 797 6 View

Which is the most recent and updated classification of Class: Insecta?

Based on the many publications from several decades, we came across many classifications of Insects. Among them which one is more updated and approved classification that can be used for research,...

05 January 2021 3,668 9 View

How can we say a species of insects got extinct?

02 January 2021 4,232 10 View

Since I am design automatized grader for forest seeds, it is important for me to know the following?

1. Is there a difference between a dormant seed, for example, of Scots pine, a fossilized dead seed, and an active live one by spectrometric or other characteristics? 2. Is it possible to...

26 November 2020 9,275 2 View

Which are the drawbacks of using a medical CT for scanning fossils?

I am in the need of scanning some fossils for analyzing their inner structure. Unfortunately I don't have access to a proper CT or micro-CT of industrial use that suits nicely fossils and neither...

16 November 2020 1,748 6 View

Is there any report of commensalic/parasitic/symbiotic relationship between fossil communities from Indian examples?

I would like to collect and understand information on commensalic/parasitic/symbiotic interactions among fossil communities from Indian geological records. if there are any reports/publications in...

14 October 2020 6,253 1 View

Could anyone provide me any useful website or bibliography to learn about the principles of taxonomy in Biology and Paleontology?

I am an undergraduate and I would like to broaden my knowledge on this subject, beginning by its principles. Maybe it would help that purpose to broaden my knowledge on Phylogenetics as well....

08 June 2020 1,343 2 View

Is it better to use base e (LN) or base 10 (LOG10) when making estimates of body mass from linear measurements?

For linear regressions between linear measurements and body mass, which transformation is better to use? base e logarithm (LN)? or base 10 logaritm (LOG10). Apparently they do not make such a for...

08 April 2020 5,201 12 View