DH technique is may be a big through in plant science if it became successful. I want to know what have been achieved using the technology and what are new ad-ons are coming into it
Doubled Haploidy (DH) has many applications in crop improvement, some of them are as follows-
1. Rapid development of homozygous inbred line in crops which show a high inbreeding depression, eg. carrot, onion etc.
2. Development of purelines varieties in self-pollinated crops in a very short period of time.
3. Development of alien substitution and/or addition lines.
4. DHs have immense use in study of recessive traits.
5. Ideal for the study of mutation frequency and spectra.
6. It helps to develop an immortal mapping population.
7. It can be used to enhance the expression level of transgene, if it is recessive.
8. Construction of genetic linkage map in DH population.
9. Identification of molecular markers linked to recessive traits or QTLs.
Currently, one of the usages of DH is on the tropical maize. See this report from CIMMYT (May 2017):
http://www.cimmyt.org/breaking-ground-vijay-chaikam-develops-doubled-haploid-lines-to-accelerate-maize-breeding/
"CIMMYT’s work on DH has greatly expanded in the past few years. Between 2012 and 2016, CIMMYT scientists produced over 100,000 DH lines, up from less than 5,000 in 2011. However, adoption of the technology is lagging behind in tropical maize breeding programs due to the lack of adapted haploid inducers with high haploid induction rates. The haploid inducers enable generations of haploids – maize varieties containing only one set of chromosomes instead of the usual two sets of chromosomes found in normal diploid maize – at a high frequency."
"......Aimed at improving the adoption of DH technology in tropical maize breeding programs by developing improved haploid inducers for tropical maize breeding programs, developing novel methods of haploid identification and efficient protocols for chromosomal doubling, and optimizing the agronomic management for deriving doubled haploids......."
Dear Satyndera
In the attached chapter, the exploitation of double haploids from classic genetic studies to contemporary plant breeding and attempt to gauge current and future impact was described.
Double haploid is a very purposeful breeding method in vegetable brassicas now a days. I can enlighten you regarding some advances in haploid production of cole crops.Like-
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